Altrenogest affects the development and endocrine milieu of ovarian follicles in prepubertal and mature gilts

• Published in: Biology of reproduction 2020-11, Vol.103 (5), p.1069-1084
• Main Authors: Ziecik, Adam J., Drzewiecka, Klaudia, Gromadzka-Hliwa, Katarzyna, Klos, Jan, Witek, Patrycja, Knapczyk-Stwora, Katarzyna, Gajewski, Zdzislaw, Kaczmarek, Monika M.
• Format: Article
• Language: English
• Subjects: altrenogest; Dosage and administration; Drug therapy; Endocrine glands; Endocrine therapy; folliculogenesis; gonadotropins; granulosa; Hogs; Ovaries; Physiological aspects; pig; Progestational agents; prostaglandins; Puberty; RESEARCH ARTICLE; Sows; steroidogenesis; theca
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1093/biolre/ioaa136

Altrenogest with gonadotropins is commonly used to synchronize the estrous cycle, but it can also lead to follicular cyst formation, especially in prepubertal gilts. Here, we aimed to investigate how maturity and altrenogest treatment affect the development, endocrine milieu, and molecular control of ovarian follicles. Crossbred prepubertal and mature gilts were challenged or not (control) with altrenogest, and ovaries were collected in the morning on the first day of behavioral estrus. In prepubertal gilts, altrenogest decreased the percentage of primordial and atretic small follicles, but increased large antral follicles when compared with controls. In mature gilts, altrenogest reduced the percentage of primary follicles and elevated the total number of antral follicles. Maturity affected the estradiol level in the follicular fluid of preovulatory follicles, luteinizing hormone (LH)-stimulated cyclic adenosine monophosphate (cAMP) generation, and LH receptor messenger RNA (mRNA) expression in granulosa. Moreover, cytochrome P45017A1 (CYP17A1) mRNA levels in the theca layer were affected and correlated with follicular androstendione and estradiol concentration. Altrenogest negatively affected follicular fluid progesterone concentration and decreased levels of prostaglandin (PG) E2 in prepubertal gilts and PGF2alpha metabolite in mature gilts. LH-stimulated cAMP release in granulosa cells of mature gilts as well as human chorionic gonadotropin- and forskolin-induced cAMP were also affected. In addition, altrenogest downregulated CYP17A1 mRNA in the prepubertal theca layer and PGF2alpha synthase expression in the granulosa and theca layer of mature gilts. To the best of our knowledge, this is the first study to report multiple effects of maturity and altrenogest on the endocrine milieu and molecular regulations governing ovarian follicle development in gilts. Summary Sentence Sexual maturity and altrenogest change endocrine milieu and molecular regulations governing folliculogenesis in gilts.