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A novel bacteriocin BM1029: physicochemical characterization, antibacterial modes and application
Aim Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work was to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechani...
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| Published in: | Journal of applied microbiology 2021-03, Vol.130 (3), p.755-768 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3539-e57b534bb5ca8d18b09a330783c870037177fdb0fd348f63e9ff2968cb945293 |
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| cites | cdi_FETCH-LOGICAL-c3539-e57b534bb5ca8d18b09a330783c870037177fdb0fd348f63e9ff2968cb945293 |
| container_end_page | 768 |
| container_issue | 3 |
| container_start_page | 755 |
| container_title | Journal of applied microbiology |
| container_volume | 130 |
| creator | Yan, H. Aizhan, R. Lu, Y.Y. Li, X. Wang, X. Yi, Y.L. Shan, Y.Y. Liu, B.F. Zhou, Y. Lü, X. |
| description | Aim
Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work was to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechanism.
Methods and Results
Bacteriocin BM1029 was purified by cation‐exchange chromatography and reversed‐phase chromatography. Antibacterial activity assay showed that BM1029 is antagonistic against both Gram‐positive and Gram‐negative bacteria. Furthermore, it was found that BM1029 showed low haemolysis with high stability to the pretreatment with different temperatures, pH and surfactants. Moreover electron microscopy and flow cytometry suggested that BM1029 inhibit indicator strains by damaging the cell envelope integrity. Cell cycle assay suggested that BM1029 arrested cell cycle in R‐phase.
Conclusion
The novel bacteriocin BM1029 showed high bactericidal activity against Escherichia coli and Staphylococcus aureus through a cell envelope‐associated mechanism.
Significance and Impact of the Study
Application of BM1029 inhibited the growth of indicator strains on beef meat storage at 4°C suggesting that this bacteriocin is promising to be used as a novel preservative in food processing and preservation. |
| doi_str_mv | 10.1111/jam.14809 |
| format | article |
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Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work was to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechanism.
Methods and Results
Bacteriocin BM1029 was purified by cation‐exchange chromatography and reversed‐phase chromatography. Antibacterial activity assay showed that BM1029 is antagonistic against both Gram‐positive and Gram‐negative bacteria. Furthermore, it was found that BM1029 showed low haemolysis with high stability to the pretreatment with different temperatures, pH and surfactants. Moreover electron microscopy and flow cytometry suggested that BM1029 inhibit indicator strains by damaging the cell envelope integrity. Cell cycle assay suggested that BM1029 arrested cell cycle in R‐phase.
Conclusion
The novel bacteriocin BM1029 showed high bactericidal activity against Escherichia coli and Staphylococcus aureus through a cell envelope‐associated mechanism.
Significance and Impact of the Study
Application of BM1029 inhibited the growth of indicator strains on beef meat storage at 4°C suggesting that this bacteriocin is promising to be used as a novel preservative in food processing and preservation.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14809</identifier><identifier>PMID: 32749036</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Antimicrobial activity ; Antimicrobial agents ; Bacteria ; Bactericidal activity ; bacteriocin ; Bacteriocins ; Bacteriocins - isolation & purification ; Bacteriocins - pharmacology ; Cattle ; Cell cycle ; Chromatography ; E coli ; Electron microscopy ; Flow cytometry ; food preservative ; Food Preservatives - isolation & purification ; Food Preservatives - pharmacology ; Food processing ; Food spoilage ; Gram-negative bacteria ; Gram-Negative Bacteria - drug effects ; Gram-Positive Bacteria - drug effects ; Lactobacillus - chemistry ; Meat ; Meat - microbiology ; mode of action ; Pollutants ; Preservatives ; Pretreatment ; Spoilage ; stability ; Strains (organisms) ; Surfactants</subject><ispartof>Journal of applied microbiology, 2021-03, Vol.130 (3), p.755-768</ispartof><rights>2020 The Society for Applied Microbiology</rights><rights>2020 The Society for Applied Microbiology.</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-e57b534bb5ca8d18b09a330783c870037177fdb0fd348f63e9ff2968cb945293</citedby><cites>FETCH-LOGICAL-c3539-e57b534bb5ca8d18b09a330783c870037177fdb0fd348f63e9ff2968cb945293</cites><orcidid>0000-0002-8624-0464</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32749036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, H.</creatorcontrib><creatorcontrib>Aizhan, R.</creatorcontrib><creatorcontrib>Lu, Y.Y.</creatorcontrib><creatorcontrib>Li, X.</creatorcontrib><creatorcontrib>Wang, X.</creatorcontrib><creatorcontrib>Yi, Y.L.</creatorcontrib><creatorcontrib>Shan, Y.Y.</creatorcontrib><creatorcontrib>Liu, B.F.</creatorcontrib><creatorcontrib>Zhou, Y.</creatorcontrib><creatorcontrib>Lü, X.</creatorcontrib><title>A novel bacteriocin BM1029: physicochemical characterization, antibacterial modes and application</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work was to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechanism.
Methods and Results
Bacteriocin BM1029 was purified by cation‐exchange chromatography and reversed‐phase chromatography. Antibacterial activity assay showed that BM1029 is antagonistic against both Gram‐positive and Gram‐negative bacteria. Furthermore, it was found that BM1029 showed low haemolysis with high stability to the pretreatment with different temperatures, pH and surfactants. Moreover electron microscopy and flow cytometry suggested that BM1029 inhibit indicator strains by damaging the cell envelope integrity. Cell cycle assay suggested that BM1029 arrested cell cycle in R‐phase.
Conclusion
The novel bacteriocin BM1029 showed high bactericidal activity against Escherichia coli and Staphylococcus aureus through a cell envelope‐associated mechanism.
Significance and Impact of the Study
Application of BM1029 inhibited the growth of indicator strains on beef meat storage at 4°C suggesting that this bacteriocin is promising to be used as a novel preservative in food processing and preservation.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bactericidal activity</subject><subject>bacteriocin</subject><subject>Bacteriocins</subject><subject>Bacteriocins - isolation & purification</subject><subject>Bacteriocins - pharmacology</subject><subject>Cattle</subject><subject>Cell cycle</subject><subject>Chromatography</subject><subject>E coli</subject><subject>Electron microscopy</subject><subject>Flow cytometry</subject><subject>food preservative</subject><subject>Food Preservatives - isolation & purification</subject><subject>Food Preservatives - pharmacology</subject><subject>Food processing</subject><subject>Food spoilage</subject><subject>Gram-negative bacteria</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Positive Bacteria - drug effects</subject><subject>Lactobacillus - chemistry</subject><subject>Meat</subject><subject>Meat - microbiology</subject><subject>mode of action</subject><subject>Pollutants</subject><subject>Preservatives</subject><subject>Pretreatment</subject><subject>Spoilage</subject><subject>stability</subject><subject>Strains (organisms)</subject><subject>Surfactants</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10E1PwyAcBnBiNE6nB7-AaeJFE7vxUgp4m4uv2eJl94ZSmrG0pcKqmZ9etk4PJnKBwO__hDwAXCA4QmGNV7IeoYRDcQBOEElpjFOGD3fnJKaQ4QE49X4FISKQpsdgQDBLBCTpCZCTqLEfuopyqdbaGatME93PEcTiLmqXG2-UVUtdGyWrSC2l69mXXBvb3EayWZv9ZHivbaF9uCsi2bZVGNmiM3BUysrr8_0-BIvHh8X0OZ69Pb1MJ7NYEUpErCnLKUnynCrJC8RzKCQhkHGiOIOQMMRYWeSwLEjCy5RoUZZYpFzlIqFYkCG47mNbZ9877ddZbbzSVSUbbTuf4YSEEIYxD_TqD13ZzjXhc0EJLCjBkAZ10yvlrPdOl1nrTC3dJkMw29aehdqzXe3BXu4Tu7zWxa_86TmAcQ8-TaU3_ydlr5N5H_kNlJWKvA</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Yan, H.</creator><creator>Aizhan, R.</creator><creator>Lu, Y.Y.</creator><creator>Li, X.</creator><creator>Wang, X.</creator><creator>Yi, Y.L.</creator><creator>Shan, Y.Y.</creator><creator>Liu, B.F.</creator><creator>Zhou, Y.</creator><creator>Lü, X.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8624-0464</orcidid></search><sort><creationdate>202103</creationdate><title>A novel bacteriocin BM1029: physicochemical characterization, antibacterial modes and application</title><author>Yan, H. ; Aizhan, R. ; Lu, Y.Y. ; Li, X. ; Wang, X. ; Yi, Y.L. ; Shan, Y.Y. ; Liu, B.F. ; Zhou, Y. ; Lü, X.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-e57b534bb5ca8d18b09a330783c870037177fdb0fd348f63e9ff2968cb945293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial activity</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bactericidal activity</topic><topic>bacteriocin</topic><topic>Bacteriocins</topic><topic>Bacteriocins - isolation & purification</topic><topic>Bacteriocins - pharmacology</topic><topic>Cattle</topic><topic>Cell cycle</topic><topic>Chromatography</topic><topic>E coli</topic><topic>Electron microscopy</topic><topic>Flow cytometry</topic><topic>food preservative</topic><topic>Food Preservatives - isolation & purification</topic><topic>Food Preservatives - pharmacology</topic><topic>Food processing</topic><topic>Food spoilage</topic><topic>Gram-negative bacteria</topic><topic>Gram-Negative Bacteria - drug effects</topic><topic>Gram-Positive Bacteria - drug effects</topic><topic>Lactobacillus - chemistry</topic><topic>Meat</topic><topic>Meat - microbiology</topic><topic>mode of action</topic><topic>Pollutants</topic><topic>Preservatives</topic><topic>Pretreatment</topic><topic>Spoilage</topic><topic>stability</topic><topic>Strains (organisms)</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, H.</creatorcontrib><creatorcontrib>Aizhan, R.</creatorcontrib><creatorcontrib>Lu, Y.Y.</creatorcontrib><creatorcontrib>Li, X.</creatorcontrib><creatorcontrib>Wang, X.</creatorcontrib><creatorcontrib>Yi, Y.L.</creatorcontrib><creatorcontrib>Shan, Y.Y.</creatorcontrib><creatorcontrib>Liu, B.F.</creatorcontrib><creatorcontrib>Zhou, Y.</creatorcontrib><creatorcontrib>Lü, X.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, H.</au><au>Aizhan, R.</au><au>Lu, Y.Y.</au><au>Li, X.</au><au>Wang, X.</au><au>Yi, Y.L.</au><au>Shan, Y.Y.</au><au>Liu, B.F.</au><au>Zhou, Y.</au><au>Lü, X.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel bacteriocin BM1029: physicochemical characterization, antibacterial modes and application</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2021-03</date><risdate>2021</risdate><volume>130</volume><issue>3</issue><spage>755</spage><epage>768</epage><pages>755-768</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work was to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechanism.
Methods and Results
Bacteriocin BM1029 was purified by cation‐exchange chromatography and reversed‐phase chromatography. Antibacterial activity assay showed that BM1029 is antagonistic against both Gram‐positive and Gram‐negative bacteria. Furthermore, it was found that BM1029 showed low haemolysis with high stability to the pretreatment with different temperatures, pH and surfactants. Moreover electron microscopy and flow cytometry suggested that BM1029 inhibit indicator strains by damaging the cell envelope integrity. Cell cycle assay suggested that BM1029 arrested cell cycle in R‐phase.
Conclusion
The novel bacteriocin BM1029 showed high bactericidal activity against Escherichia coli and Staphylococcus aureus through a cell envelope‐associated mechanism.
Significance and Impact of the Study
Application of BM1029 inhibited the growth of indicator strains on beef meat storage at 4°C suggesting that this bacteriocin is promising to be used as a novel preservative in food processing and preservation.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>32749036</pmid><doi>10.1111/jam.14809</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-8624-0464</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2021-03, Vol.130 (3), p.755-768 |
| issn | 1364-5072 1365-2672 |
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| source | Alma/SFX Local Collection |
| subjects | Animals Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antibacterial activity Antimicrobial activity Antimicrobial agents Bacteria Bactericidal activity bacteriocin Bacteriocins Bacteriocins - isolation & purification Bacteriocins - pharmacology Cattle Cell cycle Chromatography E coli Electron microscopy Flow cytometry food preservative Food Preservatives - isolation & purification Food Preservatives - pharmacology Food processing Food spoilage Gram-negative bacteria Gram-Negative Bacteria - drug effects Gram-Positive Bacteria - drug effects Lactobacillus - chemistry Meat Meat - microbiology mode of action Pollutants Preservatives Pretreatment Spoilage stability Strains (organisms) Surfactants |
| title | A novel bacteriocin BM1029: physicochemical characterization, antibacterial modes and application |
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