Extracorporeal shock wave therapy accelerates endochondral ossification and fracture healing in a rat femur delayed-union model

Extracorporeal shock wave therapy (ESWT) has been demonstrated to accelerate bone healing; however, the mechanism underlying ESWT-induced bone regeneration has not been fully elucidated. This study aimed to examine the effects of ESWT and the process of fracture healing. A rat model of femur delayed...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2020-10, Vol.530 (4), p.632-637
Main Authors: Kobayashi, Masato, Chijimatsu, Ryota, Yoshikawa, Hideki, Yoshida, Kiyoshi
Format: Article
Language:English
Subjects:
Animals
Bone Regeneration
Cell Line
Cell Proliferation
Delayed-union model
Disease Models, Animal
Endochondral ossification
Extracorporeal shock wave therapy (ESWT)
Extracorporeal Shockwave Therapy - methods
Femoral Fractures - pathology
Femoral Fractures - physiopathology
Femoral Fractures - therapy
Femur - injuries
Femur - pathology
Femur - physiopathology
Fracture Healing
Male
Mice
Osteogenesis
Rats
Rats, Sprague-Dawley
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Summary:Extracorporeal shock wave therapy (ESWT) has been demonstrated to accelerate bone healing; however, the mechanism underlying ESWT-induced bone regeneration has not been fully elucidated. This study aimed to examine the effects of ESWT and the process of fracture healing. A rat model of femur delayed-union was established by cauterizing the periosteum. ESWT treatment at the fracture site was performed 2 weeks after the operation and the site was radiographically and histologically evaluated at weeks 4, 6, and 8. The bone union rate and radiographic score of the ESWT group were significantly higher than those of the control group at 8 weeks. Histological evaluation revealed enhanced endochondral ossification at the fracture site. The effects of ESWT on ATDC5 cells were examined in vitro. ESWT promoted chondrogenic differentiation without inhibiting the proliferation of ATDC5 cells. ESWT may induce significant bone healing by promoting endochondral ossification at the fracture site. •ESWT promotes callus and bridging bone formation in the delayed-union model.•Endochondral ossification is promoted by ESWT treatment in the delayed-union model.•ESWT potentiates the chondrogenic potential of ATDC5 cells.•ESWT may promote fracture healing by enhancing the chondrogenic potential of MSCs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2020.07.084