Development of a marker vaccine candidate against classical swine fever based on the live attenuated vaccine C-strain

•Three epitope-mutated viruses were generated based on C-strain.•The mutants displayed similar phenotype and immunogenicity to C-strain.•One mutant induced antibodies distinguishable from those by C-strain. Classical swine fever (CSF) is a highly contagious and economically damaging disease. Classic...

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Published in:Veterinary microbiology 2020-08, Vol.247, p.108741-108741, Article 108741
Main Authors: Han, Yuying, Xie, Libao, Yuan, Mengqi, Ma, Yuteng, Sun, Huimin, Sun, Yuan, Li, Yongfeng, Qiu, Hua-Ji
Format: Article
Language:English
Subjects:
Amino acids
Antibodies
C-strain
Classical swine fever virus
Epitope
Epitopes
Fever
Hog cholera
Hogs
Immunization
Inoculation
Intravenous administration
Marker vaccine
Monoclonal antibodies
Mutants
Phenotypes
Spleen
Vaccines
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Summary:•Three epitope-mutated viruses were generated based on C-strain.•The mutants displayed similar phenotype and immunogenicity to C-strain.•One mutant induced antibodies distinguishable from those by C-strain. Classical swine fever (CSF) is a highly contagious and economically damaging disease. Classical swine fever virus (CSFV) lapinized vaccine C-strain against CSF worldwide lacks the capacity for the serological differentiation between infected and vaccinated animals (DIVA). To develop a marker C-strain complying with the DIVA principle, we generated and evaluated mutants rHCLV-E2F117A, rHCLV-E2G119A, and rHCLV-E2P122A, which harbor the single amino acid mutation at 117F, 119G or 122P of the monoclonal antibody HQ06-recognized epitope on the E2 glycoprotein in rabbits and pigs. Viral intravenous administration demonstrated that all the mutants retain the phenotype of C-strain in rabbits, including fever response induction and replication in the spleen. Notably, the HQ06-recognized epitope did not react with the antibodies induced by rHCLV-E2P122A in rabbits, in contrast with C-strain and other two mutants. Intramuscular administration of rHCLV-E2P122A in pigs induced anti-CSFV neutralizing antibodies but not antibodies against the HQ06-recognized epitope at 28 days post-inoculation. Collectively, our data demonstrate that rHCLV-E2P122A is a promising marker vaccine candidate against CSF.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2020.108741