First identification of a Brucella abortus biovar 4 strain from yak in Tibet, China

•We isolated the first Brucella strain XZ19-1 from yak in Tibet, China.•XZ19-1 strain belongs to B. abortus biovar 4, a biotype that has never been reported in China.•MLVA-16 genotyping revealed a novel profile (4-5-3-12-2-2-3-3-8-32-8-5-4-3-3-3) in XZ19-1 strain.•WGS-based phylogenetic analysis dem...

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Published in:Veterinary microbiology 2020-08, Vol.247, p.108751-108751, Article 108751
Main Authors: Sun, Mingjun, Liu, Mengda, Zhang, Xiyue, Zhang, Ge, Zhu, Liangquan, Ding, Jiabo, Zhang, Zhicheng, Sun, Shixiong, Sun, Shufang, Shao, Weixing, Zhang, Peipei, Zhang, Jiandong, Sun, Xiangxiang, Fan, Xuezheng, Wei, Rong
Format: Article
Language:English
Subjects:
Brucella
Brucella abortus biovar 4
Brucellosis
Epidemiology
Genomes
Genotyping
Phylogeny
Tibet
Yak
Zoonoses
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Summary:•We isolated the first Brucella strain XZ19-1 from yak in Tibet, China.•XZ19-1 strain belongs to B. abortus biovar 4, a biotype that has never been reported in China.•MLVA-16 genotyping revealed a novel profile (4-5-3-12-2-2-3-3-8-32-8-5-4-3-3-3) in XZ19-1 strain.•WGS-based phylogenetic analysis demonstrated that ZX19-1 strain was closely related to strains from European countries. Brucellosis is one of the major zoonotic diseases in the world. In China, understanding on its causative agent Brucella is still limited. Recently, we isolated a Brucella strain XZ19-1 from yak in Lhasa, Tibet. Phenotypical characterization proved that it belongs to B. abortus biovar 4, a biotype that has never been reported in China. MLVA-16 genotyping revealed a novel profile (4-5-3-12-2-2-3-3-8-32-8-5-4-3-3-3) in this strain, while MLST sequence typing demonstrated that it belongs to ST 71. Furthermore, the whole genome of XZ19-1 strain was sequenced. Subsequent phylogenetic analysis demonstrated that XZ19-1was genetically more closely related to B. abortus strains originated from European countries rather than to those collected from China previously. Isolation and identification of XZ19-1 strain may thus indicate a unique Brucella lineage existing in Qing-Tibet plateau. These findings will help to improve the diagnosis and epidemiological studies of brucellosis in animals and human in this part of China.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2020.108751