Nitric oxide plays a crucial role in midgut immunity under microsporidian infection in Antheraea pernyi

• Published in: Molecular immunology 2020-10, Vol.126, p.65-72
• Main Authors: Liu, Wei, Wang, Yong, Leng, Zheming, Wang, Qi, Duan, Xiaoxia, Luo, Yutong, Jiang, Yiren, Qin, Li
• Format: Article
• Language: English
• Subjects: Antheraeapernyi; Anti-microbial peptides; Immune; Microsporidia; Nitric oxide; Nitric oxide synthase
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/j.molimm.2020.07.018

•NO level and NOS activity were influenced by microsporidia immune stimulation.•AMP and immune genes can be influenced by NO content in Antheraea pernyi gut.•Microsporidia infection rate change under NOS inhibitor treatment. The insect gut participates in initial local immune responses by producing reactive oxygen and nitrogen species as well as anti-microbial peptides to resist pathogenic invasions. Nitric oxide (NO), a signaling and an immune effector molecule synthesized by the enzyme NO synthase (NOS), mediates an early step of the signal transduction pathway. In this study, we evaluated NO levels after Nosema pernyi infection in Antheraea pernyi gut. NOS activity was higher in the microsporidia-infected gut of A. pernyi than in that of control. Three NOS-related genes were cloned, and their spatio-temporal expression patterns were evaluated. ApNOS2 was expressed quickly in the midgut after N. pernyi infection. Sodium nitroprusside, dihydrate (SNP), or Nω-L-nitro-arginine methyl ester, hydrochloride (L-NAME), altered the NO content in A. pernyi midgut. Anti-microbial peptides (AMPs) in the groups exposed to N. pernyi plus SNP and N. pernyi plus L-NAME exhibited higher and lower expression, respectively, relative to the control. These results indicate that microsporidia infection triggers short-term activation of NO and NOS genes in the A. pernyi gut that is downregulated after 24 h. Notably, infection rates can be influenced by a NOS inhibitor. Furthermore, NO can be induced by pathogens. Similarly, NO content in the A. pernyi gut also influences AMPs in humoral immunity and some immune-related genes. Our results suggest that nitric oxide plays a vital role in A. pernyi gut immunity.