The root iron transporter 1 governs cadmium uptake in Vicia sativa roots

• Published in: Journal of hazardous materials 2020-11, Vol.398, p.122873-122873, Article 122873
• Main Authors: Zhang, Xingxing, Li, Xin, Tang, Ling, Peng, Yizhe, Qian, Meng, Guo, Yafang, Rui, Haiyun, Zhang, Fenqin, Hu, Zhubing, Chen, Yahua, Xia, Yan, Shen, Zhenguo
• Format: Article
• Language: English
• Subjects: Cadmium - toxicity; Competitive uptake; Iron; Plant Roots; Root iron transporter 1,Cd toxicity; Transient Cd influx; Vicia sativa; Zinc
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2020.122873

[Display omitted] •The ZM variety of V. sativa showed more Cd-accumulation than L3.•The expression level of VsRIT1 was higher in ZM than L3 under Cd toxicity.•Three differential amino acid residues in VsRIT1 from ZM and L3 is firstly observed.•VsRIT1 participates in cadmium uptake in V. sativa roots. Cadmium is a non-essential element for plants and that inhibits plant growth and development. The Zhangye Mawan (ZM) variety of Vicia sativa is more sensitive to Cd toxicity than that Lanjian 3# (L3) variety, but the underlying mechanism is not fully understood. Here, we demonstrated that ZM showed higher Cd accumulation than L3 based on root Cd content and Cd fluorescence intensity in root protoplasts. VsRIT1, a member of the ZIP (ZRT/IRT-like protein) family, showed expression levels in ZM roots 8-fold higher than those in L3 roots under Cd exposure. VsRIT1 expression increased Cd transport and accumulation in Arabidopsis and yeast. These suggests that VsRIT1 participates in Cd uptake by V. sativa roots. Furthermore, ZM root tips have a higher capacity for transient Cd influx than L3 roots when exposed to Cd alone or Cd and iron (Fe) together, owing to the higher VsRIT1 expression in ZM. Our findings also imply that Cd may compete with Fe or/and zinc (Zn) for uptake via VsRIT1 in V. sativa or yeast.