Preconditioning adipose-derived stem cells with photobiomodulation significantly increased bone healing in a critical size femoral defect in rats

• Published in: Biochemical and biophysical research communications 2020-10, Vol.531 (2), p.105-111
• Main Authors: Khosravipour, Armin, Amini, Abdollah, Masteri Farahani, Reza, Zare, Fatemeh, Mostafavinia, Atarodsadat, Fallahnezhad, Somaye, Akbarzade, Saman, Ava parvandi, Asgari, Mehrdad, Mohammadbeigi, Ahmad, Rezaei, Fatemehsadat, Ghoreishi, Seyed Kamran, Chien, Sufan, Bayat, Mohammad
• Format: Article
• Language: English
• Subjects: Adipose Tissue - cytology; Animals; Biomarkers - metabolism; Biomechanical Phenomena; Bone Matrix - radiation effects; Bone Matrix - ultrastructure; Cell Survival - radiation effects; Critical size bone defect; Demineralized bone scaffold; Elastic Modulus; Femur - pathology; Femur - radiation effects; Fracture healing; Human adipose-derived stem cell; Humans; Low-Level Light Therapy; Male; Photobiomodulation; Rats, Wistar; Stem Cells - cytology; Stem Cells - radiation effects; Wound Healing - radiation effects
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2020.07.048

We assessed the combined impacts of human demineralized bone matrix (hDBM) scaffold, adipose-derived stem cells (hADS), and photobiomodulation (PBM) on bone repair of a critical size femoral defect (CSFD) in 72 rats. The rats were divided into six groups: control (group 1); ADS (group 2 - ADS transplanted into hDBM); PBM (group 3 - PBM-treated CSFDs); ADS + PBM in vivo (group 4 - ADS transplanted into hDBM and the CSFDs were treated with PBM in vivo); ADS + PBM in vitro (group 5 - ADS were treated with PBM in vitro, then seeded into hDBM); and ADS + PBM in vitro+in vivo (group 6 - PBM-treated ADS were seeded into hDBM, and the CSFDs were treated with PBM in vivo. At the anabolic phase (2 weeks after surgery), bone strength parameters of the groups 5, 6, and 4 were statistically greater than the control, ADS, and PBM in vivo groups (all, p = 0.000). Computed tomography (CT) scans during the catabolic phase (6 weeks after surgery) of bone healing revealed that the Hounsfield unit (HU) of CSFD in the groups 2 (p = 0.000) and 5 (p = 0.019) groups were statistically greater than the control group. The groups 5, 4, and 6 had significantly increased bone strength parameters compared with the PBM in vivo, control, and ADS groups (all, p = 0.000). The group 5 was statistically better than the groups 4, and 6 (both, p = 0.000). In vitro preconditioned of hADS with PBM significantly increased bone repair in a rat model of CSFD in vivo. •In vitro preconditioned of adipose derived stem cells (ADS) with photobiomodulation (PBM) increased cell viability.•A combination of ADS plus PBM increased bone repair of repairing tissue in the large bone defect.•ADS preconditioned with PBM in vitro, increased bone repair in vivo.•ADS preconditioned with PBM in vitro presented better results than other protocols.