Functional analysis of PGI1 and ZWF1 in thermotolerant yeast Kluyveromyces marxianus

• Published in: Applied microbiology and biotechnology 2020-09, Vol.104 (18), p.7991-8006
• Main Authors: Zhang, Biao, Ren, Lili, Zeng, Shuai, Zhang, Siyang, Xu, Dayong, Zeng, Xin, Li, Feng
• Format: Article
• Language: English
• Subjects: Applied Microbial and Cell Physiology; Biomedical and Life Sciences; Biotechnology; Catabolite repression; Chemical synthesis; Dextrose; Ethanol; Fermentation; Functional analysis; Gene expression; Glucose; Glucose metabolism; Glycolysis; Kluyveromyces - genetics; Kluyveromyces marxianus; Life Sciences; Metabolic pathways; Metabolism; Microbial Genetics and Genomics; Microbiology; Mutants; NADH; NADP; Nicotinamide adenine dinucleotide; Pentose; Pentose phosphate pathway; Phosphates; Phosphoglucose isomerase; Physiological aspects; Plant biomass; Public-private sector cooperation; Raw materials; Saccharomyces cerevisiae; Xylose; Yeast; Yeasts
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-020-10808-4

Glycolysis and the pentose phosphate pathway (PPP) are two basic metabolic pathways that are simultaneously present in yeasts. As the main pathway in most species, the glycolysis provides ATP and NADH for cell metabolism while PPP, as a complementary pathway, supplies NADPH. In this study, the performance of Kluyveromyces marxianus using glycolysis or PPP were studied through the disruption of PGI1 or ZWF1 gene, respectively. K. marxianus using glycolysis as the only pathway showed higher ethanol production ability than that of the Kluyveromyces lactis zwf1Δ mutant; K. marxianus using only PPP showed more robustness than that of Saccharomyces cerevisiae pgi1Δ mutant. Additionally, K. marxianus pgi1Δ strain accumulated much more intracellular NADPH than the wild type strain and co-utilized glucose and xylose more effectively. These findings suggest that phosphoglucose isomerase participates in the regulation of the repression of glucose on xylose utilization in K. marxianus . The NADPH/NADP + ratio, dependent on the activity of the PPP, regulated the expression of multiple genes related to NADPH metabolism in K. marxianus (including NDE1 , NDE2 , GLR1 , and GDP1 ). Since K. marxianus is considered a promising host in industrial biotechnology to produce renewable chemicals from plant biomass feedstocks, our research showed the potential of the thermotolerant K. marxianus to produce NADP(H)-dependent chemical synthesis from multiple feedstocks. Key points • The function of PGI1 and ZWF1 in K. marxianus has been analyzed in this study. • K. marxianus zwf1Δ strain produced ethanol but with decreased productivity. • K. marxianus pgi1Δ strain grew with glucose and accumulated NADPH. • K. marxianus pgi1Δ strain released glucose repression on xylose utilization.