Evaluation of autophagy‐related genes in Egyptian systemic lupus erythematosus patients

Disturbances in autophagy are known to be implicated in autoimmune disorders. Many studies have connected polymorphisms in autophagy‐related gene 5 (ATG‐5) to systemic lupus erythematosus (SLE). Our aim was the determination of the expression level of ATG‐5, Beclin‐1 and microtubule‐associated prote...

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Bibliographic Details
Published in:International journal of rheumatic diseases 2020-08, Vol.23 (9), p.1226-1232
Main Authors: Kamel, Ayat M., Badary, Mohamed S., Mohamed, Wegdan A., Ahmed, Ghada H., El‐Feky, Mohamed A.
Format: Article
Language:English
Subjects:
Alleles
ATG
Autoimmune diseases
Autophagy
Beclin‐1
Complement component C3
Complement component C4
Gene polymorphism
Genes
LC‐3
Lupus
Phagocytosis
Polymorphism
Single-nucleotide polymorphism
SLE
Systemic lupus erythematosus
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Summary:Disturbances in autophagy are known to be implicated in autoimmune disorders. Many studies have connected polymorphisms in autophagy‐related gene 5 (ATG‐5) to systemic lupus erythematosus (SLE). Our aim was the determination of the expression level of ATG‐5, Beclin‐1 and microtubule‐associated protein‐light chain 3 (LC‐3) in Egyptian SLE patients to investigate the impact of disturbances in autophagy genes on the incidence and progression of the disease. Also, we investigated the incidence of single nucleotide polymorphism (SNP) rs573775 in ATG‐5 gene among Egyptian SLE patients. Our results showed that the mean levels of Beclin‐1, LC‐3 and interleukin (IL)‐10 transcripts were significantly higher in SLE patients compared to healthy controls. The previous transcripts were positively correlated with SLE Disease Activity Index (SLEDAI). Beclin‐1 and LC‐3 transcripts were negatively correlated to complement component 3 (C3) levels. Only LC‐3 transcripts were negatively correlated to complement component 4 (C4). The rs573775 SNP of ATG‐5 with the variant allele was significantly associated with disease susceptibility, conferring a higher risk of SLE development. This variant allele was more prevalent in patients below 30 years, patients with anemia and in patients with anti‐double‐stranded DNA (dsDNA), confirming the essential role of ATG‐5 polymorphism in the susceptibility of Egyptian patients to SLE.
ISSN:1756-1841
1756-185X
DOI:10.1111/1756-185X.13910