Flow cytometric analysis of platelet function to improve the recognition of thrombocytopathy

Light transmission aggregometry (LTA) is the gold standard for diagnosing bleeding disorders. Although LTA is laborious, requires large volumes of blood and is relatively insensitive to small changes in platelet function, there is still no competing alternative approach to replace LTA for the diagno...

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Bibliographic Details
Published in:Thrombosis research 2020-10, Vol.194, p.183-189
Main Authors: Huskens, Dana, Li, Li, Florin, Lisa, de Kesel, Pieter, de Laat, Bas, Roest, Mark, Devreese, Katrien M.J.
Format: Article
Language:English
Subjects:
Aggregometry
Flow cytometry
Platelet function defect
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Summary:Light transmission aggregometry (LTA) is the gold standard for diagnosing bleeding disorders. Although LTA is laborious, requires large volumes of blood and is relatively insensitive to small changes in platelet function, there is still no competing alternative approach to replace LTA for the diagnosis of platelet bleeding disorders. This study investigates the correlation between flow cytometry-based whole blood platelet activation test (WB-PACT) and LTA and whether WB-PACT is of additional value for the identification of bleeding disorders. In total, 161 patients with suspected bleeding diathesis were tested. A correlation of 0.41 between LTA and WB-PACT was found, and there was agreement between tests in 62% of cases (κ = 0.23). The WB-PACT is of additional value to LTA to detect platelet function disorders (PFD) as 10 patients with elevated bleeding score (BS) were detected with WB-PACT, 4 with LTA and 7 patients were positive with both tests. Interestingly, in contrast to LTA, WB-PACT has an additional option to detect VWF disfunctions. WB-PACT may have added value for the routine diagnostic work-up in patients who need to have platelet function tested. •Flow cytometric analysis of platelet function by WB-PACT seems to improve the recognition of PFD in patients with bleeding diathesis.•VWF function application of the WB-PACT test seems superior to the ristocetin induced agglutination measurements for detecting VWF disfunction in patients with bleeding diathesis.•Advantages of WB-PACT, in comparison with LTA, are the requirement of minimal amounts of blood and the reduction on labor and time.
ISSN:0049-3848
1879-2472
DOI:10.1016/j.thromres.2020.06.037