Genome-guided insights of tropical Bacillus strains efficient in maize growth promotion

• Published in: FEMS microbiology ecology 2020-09, Vol.96 (9), p.1
• Main Authors: Vieira Velloso, Camila Cristina, de Oliveira, Christiane Abreu, Gomes, Eliane Aparecida, Lana, Ubiraci Gomes de Paula, de Carvalho, Chainheny Gomes, Guimarães, Lauro José Moreira, Pastina, Maria Marta, de Sousa, Sylvia Morais
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Bacillus; Bacillus (Bacteria); Bacillus - genetics; Bacteria; Biofilms; Corn; Dry weight; Ecology; Environmental aspects; Exopolysaccharides; Genetic aspects; Genomes; Genomic analysis; Genomics; Genotypes; Growth; Inoculation; Microbiology; Morphology; Nucleotide sequence; Osmotic stress; Phytase; Plant Development; Plant growth; Plant Roots; Polyethylene glycol; Seedlings; Strains (organisms); Weight; Zea mays
• ISSN: 1574-6941; 0168-6496; 1574-6941
• DOI: 10.1093/femsec/fiaa157

ABSTRACT Plant growth promoting bacteria (PGPB) are an efficient and sustainable alternative to mitigate biotic and abiotic stresses in maize. This work aimed to sequence the genome of two Bacillus strains (B116 and B119) and to evaluate their plant growth-promoting (PGP) potential in vitro and their capacity to trigger specific responses in different maize genotypes. Analysis of the genomic sequences revealed the presence of genes related to PGP activities. Both strains were able to produce biofilm and exopolysaccharides, and solubilize phosphate. The strain B119 produced higher amounts of IAA-like molecules and phytase, whereas B116 was capable to produce more acid phosphatase. Maize seedlings inoculated with either strains were submitted to polyethylene glycol-induced osmotic stress and showed an increase of thicker roots, which resulted in a higher root dry weight. The inoculation also increased the total dry weight and modified the root morphology of 16 out of 21 maize genotypes, indicating that the bacteria triggered specific responses depending on plant genotype background. Maize root remodeling was related to growth promotion mechanisms found in genomic prediction and confirmed by in vitro analysis. Overall, the genomic and phenotypic characterization brought new insights to the mechanisms of PGP in tropical Bacillus. Tropical Bacillus strains have plant growth-promoting characteristics and genes related to nutrient uptake, phytohormone production and protection against environmental stresses.