NHEJ inhibitor SCR7 and its different forms: Promising CRISPR tools for genome engineering

The CRISPR-Cas system currently stands as one of the best multifaceted tools for site-specific genome engineering in mammals. An important aspect of research in this field focusses on improving the specificity and efficacy of precise genome editing in multiple model systems. The cornerstone of this...

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Bibliographic Details
Published in:Gene 2020-12, Vol.763, p.144997-144997, Article 144997
Main Authors: Ray, Ujjayinee, Vartak, Supriya V., Raghavan, Sathees C.
Format: Article
Language:English
Subjects:
Double-strand break
DSB Repair
Genome editing
Immunodeficiency
Ligase IV
Nonhomologous DNA end joining
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Summary:The CRISPR-Cas system currently stands as one of the best multifaceted tools for site-specific genome engineering in mammals. An important aspect of research in this field focusses on improving the specificity and efficacy of precise genome editing in multiple model systems. The cornerstone of this mini-review is one of the extensively investigated small molecule inhibitor, SCR7, which abrogates NHEJ, a Ligase IV-dependent DSB repair pathway, thus guiding integration of the foreign DNA fragment via the more precise homology directed repair during genome editing. One of our recent studies sheds light on properties of different forms of SCR7. Here, we give a succinct account on the use of SCR7 and its different forms in CRISPR-Cas system, highlighting their chemical properties and biological relevance as potent efficiency-enhancing CRISPR tools.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2020.144997