Transcriptional profiling reveals molecular basis and the role of arginine in response to low-pH stress in Pichia kudriavzevii

The non-conventional yeast Pichia kudriavzevii is considered to be a promising biotechnological host for the production of organic acids under low-pH conditions. However, little is known about the low-pH stress response in P. kudriavzevii, which significantly restricts its future development. In thi...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2020-12, Vol.130 (6), p.588-595
Main Authors: Ji, Hao, Xu, Ke, Dong, Xiameng, Sun, Da, Peng, Renyi, Lin, Sue, Zhang, Kailun, Jin, Libo
Format: Article
Language:English
Subjects:
Arginine
Arginine - metabolism
Cell aggregation
Fermentation
Hydrogen-Ion Concentration
Low-pH
Oxidative Stress - genetics
Pichia - genetics
Pichia - metabolism
Pichia kudriavzevii
RNA-Seq
Transcription, Genetic
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Summary:The non-conventional yeast Pichia kudriavzevii is considered to be a promising biotechnological host for the production of organic acids under low-pH conditions. However, little is known about the low-pH stress response in P. kudriavzevii, which significantly restricts its future development. In this study, P. kudriavzevii N-X showed great tolerance to low-pH stress, but the cell aggregation upon extremely acidic conditions might be unfavorable for low-pH fermentation. We therefore conducted RNA-Seq to compare global gene expression of P. kudriavzevii N-X in response to different pH stresses. Totally 434 genes were identified to be differentially expressed genes (DEGs), and annotation and enrichment analysis suggested that multiple genes associated with regulation of membrane lipid composition, filamentous growth and arginine metabolism were differentially expressed. The increased specific activity of arginase and intracellular ammonia concentration of P. kudriavzevii cultured at pH 2.0 further implied potential roles of arginine in response to extreme low-pH conditions. Extracellular supplementation of 5 mM arginine resulted in increased pHi and cell growth at pH 2.0, meanwhile the cell aggregation was partially suppressed. Additionally, overexpression of ARG J involving in arginine synthesis can also enhance the cell growth and reduce the aggregation effect. These results suggested that increasing arginine flux might be an alternative approach in the developing of P. kudriavzevii as a platform host for production of organic acids under low-pH conditions.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2020.07.014