Exosomes Derived From HEK293T Cells Interact in An Efficient and Noninvasive Manner with Mammalian Sperm In Vitro

Aim: To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2020-08, Vol.15 (20), p.1965-1980
Main Authors: Vilanova-Perez, Teresa, Jones, Celine, Balint, Stefan, Dragovic, Rebecca, L Dustin, Michael, Yeste, Marc, Coward, Kevin
Format: Article
Language:English
Subjects:
Antibodies
Infertility
Manufacturing
Nanoparticles
Proteins
Sperm
Transmission electron microscopy
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Summary:Aim: To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-h of exposure to exosomes, there were no significant deleterious effects with regard to sperm motility, viability, membrane integrity and mitochondrial membrane potential (p > 0.05), thus indicating that exosomes did not interfere with basic sperm function. Conclusion: HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2020-0056