Exosomes Derived From HEK293T Cells Interact in An Efficient and Noninvasive Manner with Mammalian Sperm In Vitro

Aim: To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis...

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Published in:Nanomedicine (London, England) England), 2020-08, Vol.15 (20), p.1965-1980
Main Authors: Vilanova-Perez, Teresa, Jones, Celine, Balint, Stefan, Dragovic, Rebecca, L Dustin, Michael, Yeste, Marc, Coward, Kevin
Format: Article
Language:English
Subjects:
Antibodies
Infertility
Manufacturing
Nanoparticles
Proteins
Sperm
Transmission electron microscopy
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cited_by cdi_FETCH-LOGICAL-c2526-4c458d432304827296dd6f71be58d75c19587a4fbef0499c9e731a3e2999f94c3
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container_end_page 1980
container_issue 20
container_start_page 1965
container_title Nanomedicine (London, England)
container_volume 15
creator Vilanova-Perez, Teresa
Jones, Celine
Balint, Stefan
Dragovic, Rebecca
L Dustin, Michael
Yeste, Marc
Coward, Kevin
description Aim: To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-h of exposure to exosomes, there were no significant deleterious effects with regard to sperm motility, viability, membrane integrity and mitochondrial membrane potential (p > 0.05), thus indicating that exosomes did not interfere with basic sperm function. Conclusion: HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.
doi_str_mv 10.2217/nnm-2020-0056
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subjects Antibodies
Infertility
Manufacturing
Nanoparticles
Proteins
Sperm
Transmission electron microscopy
title Exosomes Derived From HEK293T Cells Interact in An Efficient and Noninvasive Manner with Mammalian Sperm In Vitro
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