Impact of Intestinal Microbiota on Reconstitution of Circulating Monocyte, Dendritic Cell, and Natural Killer Cell Subsets in Adults Undergoing Single-Unit Cord Blood Transplantation

• Published in: Biology of blood and marrow transplantation 2020-11, Vol.26 (11), p.e292-e297
• Main Authors: Konuma, Takaaki, Kohara, Chisato, Watanabe, Eri, Takahashi, Shunsuke, Ozawa, Genki, Inomata, Kentaro, Suzuki, Kei, Mizukami, Motoko, Nagai, Etsuko, Okabe, Motohito, Isobe, Masamichi, Kato, Seiko, Oiwa-Monna, Maki, Takahashi, Satoshi, Tojo, Arinobu
• Format: Article
• Language: English
• Subjects: Cord blood transplantation; Dendritic cell; Immune reconstitution; Intestinal microbiota; Monocyte; Natural killer cell
• ISSN: 1083-8791; 1523-6536
• DOI: 10.1016/j.bbmt.2020.08.009

•A greater diversity of intestinal microbiota at 1 month was significantly associated with higher counts of plasmacytoid dendritic cells at 7 months after single-unit cord blood transplantation (CBT).•The families Neisseriaceae, Burkholderiaceae, Propionibacteriaceae, and Coriobacteriaceae were increased in higher classical monocyte reconstitution at 7 months post-CBT, whereas the family Bacteroidaceae was increased in lower classical monocyte reconstitution at 7 months post-CBT.•Intestinal microbiota composition affected immune reconstitution of classical monocyte and plasmacytoid dendritic cells following single-unit CBT. The intestinal microbiota plays a fundamental role in the development of host innate immune cells, such as monocytes, dendritic cells (DCs), and natural killer (NK) cells. We examined the association between intestinal microbiota and subsequent immune reconstitution of circulating monocyte, DC, and NK cell subsets in 38 adult patients undergoing single-unit cord blood transplantation (CBT). A higher diversity of intestinal microbiota at 1 month was significantly associated with higher counts of plasmacytoid DCs at 7 months after CBT, as measured by the Chao1 index. Principal coordinate analysis of unweighted UniFrac distances showed significant differences between higher and lower classical monocyte reconstitution at 7 months post-CBT. The families Neisseriaceae, Burkholderiaceae, Propionibacteriaceae, and Coriobacteriaceae were increased in higher classical monocyte reconstitution at 7 months post-CBT, whereas the family Bacteroidaceae was increased in lower classical monocyte reconstitution at 7 months post-CBT. These data show that intestinal microbiota composition affects immune reconstitution of classical monocyte and plasmacytoid DCs following single-unit CBT.