“Sporotan” a new fluorescent stain for identifying cryptic spores of Rhodobacter johrii

We propose a new fluorescent stain “sporotan” and staining protocol which aid in the identification of cryptic endospores which are otherwise mistaken as poly-β-hydroxyalkanoate granules. •We propose a new bacterial endospore specific fluorescent stain to identify spores produced by Rhodobacter johr...

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Bibliographic Details
Published in:Journal of microbiological methods 2020-10, Vol.177, p.106019-106019, Article 106019
Main Authors: Ashif Ali, Gaurav, Kumar, Senthilnathan, N., Radhakrishnan, T.P., Sasikala Ch, Ramana, Ch.V.
Format: Article
Language:English
Subjects:
Endospores
Fluorescent stain
Proteobacterium
Rhodobacter johrii
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Summary:We propose a new fluorescent stain “sporotan” and staining protocol which aid in the identification of cryptic endospores which are otherwise mistaken as poly-β-hydroxyalkanoate granules. •We propose a new bacterial endospore specific fluorescent stain to identify spores produced by Rhodobacter johrii.•“Sporotan” chemically is 7,7-bis(n-hexylamino)-8,8-dicyanoquinodimethane•The staining protocol is very simple, easy, reliable, specific and reproducible.•Helps in the identification of spore producers among members of phyla other than of the phylum Firmicutes.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2020.106019