Tannic acid-reinforced methacrylated chitosan/methacrylated silk fibroin hydrogels with multifunctionality for accelerating wound healing

• Published in: Carbohydrate polymers 2020-11, Vol.247, p.116689-116689, Article 116689
• Main Authors: He, Xi, Liu, Xuezhe, Yang, Jin, Du, Haibo, Chai, Ningwen, Sha, Zhou, Geng, Mengru, Zhou, Xiaojun, He, Chuanglong
• Format: Article
• Language: English
• Subjects: Adhesiveness; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antioxidants - chemistry; Antioxidants - pharmacology; Antioxidants - therapeutic use; Bandages; Cell Proliferation - drug effects; Chitosan - chemistry; Chitosan - pharmacology; Chitosan - therapeutic use; Cross-Linking Reagents - chemistry; Escherichia coli - drug effects; Fibroins - chemistry; Fibroins - pharmacology; Fibroins - therapeutic use; Hydrogels - chemical synthesis; Hydrogels - chemistry; Hydrogels - pharmacology; Hydrogels - therapeutic use; Mechanical Phenomena; Methacrylated chitosan; Methacrylated silk fibroin; Methacrylates - chemistry; Mice; Mice, Inbred ICR; Multifunctional hydrogel; NIH 3T3 Cells; Rheology; Staphylococcus aureus - drug effects; Tannic acid; Tannins - chemistry; Tannins - pharmacology; Tannins - therapeutic use; Ultraviolet Rays; Wound dressing; Wound Healing - drug effects; Wounds and Injuries - pathology; Wounds and Injuries - therapy
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.116689

[Display omitted] •TA-reinforced CSMA/SFMA hydrogels with double-network were developed.•TA-reinforced hydrogels exhibited enhanced mechanical properties.•Adhesiveness, antibacterial and antioxidative properties were improved by TA treatment.•TA-reinforced hydrogels significantly accelerated the wound healing process. Natural polymeric hydrogel featuring multifunctional properties is more attractive as wound dressing. Herein, Tannic acid (TA)-reinforced methacrylated chitosan (CSMA)/methacrylated silk fibroin (SFMA) hydrogels were fabricated by two-step method of photopolymerization and TA solution incubating treatment. The TA in hydrogels not only served as second crosslinker improving the mechanical performance of up to a 5-fold increase (5 % TA treatment) than the pristine one, but also as functional molecule that endowed the hydrogels with enhanced adhesiveness and antioxidative properties. Besides, the introduction of TA into hydrogels further improved the antimicrobial activities against both Escherichia coli (E. coli) and Staphylococcus Aureus (S. aureus), as well as the cytocompatibility on fibroblasts. Moreover, it was demonstrated that the TA-treated CSMA/SFMA hydrogels could significantly promote wound healing in a full-thickness skin defect model. Collectively, these results showed that TA-reinforced CSMA/SFMA hydrogels could be a promising candidate as wound dressing.