Size exclusion chromatography: An effective tool for the estimation of Sirolimus levels

•New method for the estimation Sirolimus levels.•LoQ is 0.11 μg/ml of standard solution.•Convenient and easy methodology. Sirolimus, a lipophilic macrolide, is a well-known immunosuppressant drug used for coating coronary stents and for preventing rejection of kidney transplants in humans. Since Sir...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2020-11, Vol.1156, p.122303-122303, Article 122303
Main Authors: Sreejith, Sasidharan L., Aswathy, Babu P., Joseph, Roy, Sasi, Renjith
Format: Article
Language:English
Subjects:
Analysis
Chromatography
Chromatography, Gel - methods
Chromatography, High Pressure Liquid
Extraction
Humans
Limit of Detection
Linear Models
Reproducibility of Results
Sirolimus
Sirolimus - blood
Size exclusion chromatography
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Summary:•New method for the estimation Sirolimus levels.•LoQ is 0.11 μg/ml of standard solution.•Convenient and easy methodology. Sirolimus, a lipophilic macrolide, is a well-known immunosuppressant drug used for coating coronary stents and for preventing rejection of kidney transplants in humans. Since Sirolimus is a relatively large molecule with an average mass 914.172 g/mol, size exclusion chromatography (SEC) was employed for exploring its potential for the estimation of Sirolimus content from blood samples. When human blood samples were spiked with known concentrations of Sirolimus, it was observed that it could be estimated with an average recovery of >90% and relative standard deviation
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2020.122303