Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata

• Published in: International journal of biological macromolecules 2020-12, Vol.164, p.3421-3428
• Main Authors: Gao, Na, Chen, Ru, Mou, Rongrong, Xiang, Jingying, Zhou, Kai, Li, Zi, Zhao, Jinhua
• Format: Article
• Language: English
• Subjects: Animals; Anticoagulant; Anticoagulants - chemistry; Anticoagulants - isolation & purification; Anticoagulants - pharmacology; Chemical Fractionation; Chemical Phenomena; Chemical structure; Chondroitin Sulfates; Chromatography, High Pressure Liquid; Fucosylated chondroitin sulfate; Holothuria - chemistry; Magnetic Resonance Spectroscopy; Molecular Structure; Polysaccharides - chemistry; Polysaccharides - pharmacology; Sea cucumber; Sea Cucumbers - chemistry; Sulfated aminoglycan; Sulfated fucan; Sulfates - chemistry
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.08.150

Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities. In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization. They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses. The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively. Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis. As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3−)-α1→, respectively. The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→. The sulfated AG was composed of four types of monosaccharides. Their anticoagulant activities were further evaluated in vitro. FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity. These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality. •Four sulfated polysaccharides were purified from the sea cucumber H. fuscopunctata.•The physicochemical properties of these polysaccharides were characterized.•The depolymerization methods could facilitate to separate sulfated polysaccharides.•The anticoagulant activities of these polysaccharides were evaluated.