Pathogenicity of Campylobacter strains of poultry and human origin from Poland

• Published in: International journal of food microbiology 2020-12, Vol.334, p.108830-108830, Article 108830
• Main Authors: Wysok, Beata, Wojtacka, Joanna, Kivistö, Rauni
• Format: Article
• Language: English
• Subjects: Adhesion; Campylobacter; Cell line; Cytotoxicity; FlaA protein; flaA-SVR sequencing; Genes; Inoculum; Invasion; Invasiveness; Pathogenicity; Pathogens; Poultry; Reduction; Strains (organisms); Toxicity; Virulence; Virulence factor
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2020.108830

The aim of this study was to determine the pathogenic markers associated with Campylobacter infection in humans. A total of 104 Campylobacter isolates obtained from poultry and humans were examined for the presence of nine virulence genes and their ability to adhere to, invade and produce cytotoxin were defined using HeLa cells. The diversity of the Campylobacter spp. isolates was studied based on sequencing of the SVR-region of flaA gene. Altogether 45 flaA-SVR alleles were identified among 104 Campylobacter isolates of poultry and human origin. All Campylobacter isolates possessed flaA, cadF and racR genes involved in adherence. Accordingly, all poultry and human isolates exhibited adherence towards HeLa cells at mean levels of 0.95% and 0.82% of starting viable inoculum, respectively. The genes involved in invasion (iam and pldA) and cytotoxin production (cdtA, cdtB and cdtC) were also widely distributed among the human and poultry Campylobacter isolates. Significantly higher invasiveness was observed for poultry isolates (mean level of 0.002% of starting bacterial inoculum) compared to human isolates (0.0005%). Interestingly the iam gene, associated with invasion, was more common in human (100%) than poultry (84%) isolates, and the poultry isolates lacking the iam gene showed a marked reduction in their ability to invade HeLa cells. Moreover, virB11 was present in 22% of the poultry and 70.4% of the human isolates. Strains lacking virB11 showed a slight reduction in invasion, however in the absence of iam even the poultry isolates containing virB11 were unable to invade HeLa cells. The mean cytotoxicity of Campylobacter isolates from poultry and human was 26.7% and 38.7%, respectively. Strains missing both the cdtB and cdtC genes were non-cytotoxic compared to strains containing all three cdtABC genes, which were the most cytotoxic among the C. jejuni and C. coli isolates from both sources. No cytotoxic effect was observed in only 4% of poultry and 5.6% of human isolates. •Campylobacter isolates from Polish poultry and humans showed high genetic diversity and overlapping flaA-SVR alleles.•Genes associated with virulence were widely dispersed in both sources.•Both isolates from poultry and humans exhibited similar levels of cytotoxicity and adhesion abilities.•Poultry isolates with a similar gene pattern to the human isolates showed higher levels of invasion.•Isolates lacking the iam gene showed a marked reduction in their ability to invade epithelial c