Identification and characterization of Pseudozyma antarctica Δ12 fatty acid desaturase and its utilization for the production of polyunsaturated fatty acids

Fatty acid desaturases, especially Δ12 fatty acid desaturases, are key enzymes for the production of unsaturated fatty acids in oleaginous yeasts. In this study, we identified and characterized a gene encoding Δ12 fatty acid desaturase of Pseudozyma antarctica named PaFAD2. Almost all oleic acid (C1...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2020-12, Vol.130 (6), p.604-609
Main Authors: Matsuzawa, Tomohiko, Maehara, Tomoko, Kamisaka, Yasushi, Ayabe-Chujo, Yuko, Takaku, Hiroaki, Yaoi, Katsuro
Format: Article
Language:English
Subjects:
Basidiomycota - enzymology
Fatty acid desaturase
Fatty Acid Desaturases - genetics
Fatty Acid Desaturases - metabolism
Fatty Acids, Unsaturated - biosynthesis
Gene Expression
Lipomyces starkeyi
Oleaginous yeast
Polyunsaturated fatty acids
Pseudozyma antarctica
Saccharomyces cerevisiae
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Summary:Fatty acid desaturases, especially Δ12 fatty acid desaturases, are key enzymes for the production of unsaturated fatty acids in oleaginous yeasts. In this study, we identified and characterized a gene encoding Δ12 fatty acid desaturase of Pseudozyma antarctica named PaFAD2. Almost all oleic acid (C18:1) was converted to linoleic acid by the heterologous expression of the PaFAD2 gene in Saccharomyces cerevisiae and Lipomyces starkeyi oleaginous yeast. Notably, PaFad2 converted not only oleic acid to linoleic acid, but also palmitoleic acid (C16:1) to 9,12-hexadecadienoic acid (C16:2). These results indicated that the PaFAD2 gene was very useful for the production of polyunsaturated fatty acids in yeast, including oleaginous yeast.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2020.07.019