Controlled release of sex steroids through osmotic pump alters brain GnRH1 and catecholaminergic system dimorphically in the catfish, Clarias gariepinus

• Published in: Brain research bulletin 2020-11, Vol.164, p.325-333
• Main Authors: Mamta, Sajwan-Khatri, Sudhakumari, Chenichery, Kagawa, Hirohiko, Dutta-Gupta, Aparna, Senthilkumaran, Balasubramanian
• Format: Article
• Language: English
• Subjects: Brain-Pituitary-Gonadal Axis; Dopamine; GnRH1; Osmotic Pump; Sex steroids; Teleosts
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2020.08.022

•Controlled release of sex steroids modulates GnRH and CAs activity dimorphically in catfish.•Brain-related transcripts elevated after estrogenization as compared to androgenization.•Estrogen mediated feminization of male brain is intense. The present study aimed to evaluate osmotic pump-mediated controlled release of estrogen in males and androgen in females to analyze the impact on gonadotropin-releasing hormone (GnRH1), catecholamines (CAs) and other associated genes in the catfish, Clarias gariepinus. During pre-spawning phase, catfish were separately implanted osmotic pumps loaded with 17β-estradiol (E2) in males and 17α-methyltestosterone (MT) in females at a dose of 10 μg/100 μl or saline (100 μl) controls into both sexes to release for 21 days and all fishes were maintained as per the duration. Further, GnRH1 expression levels were analysed in the discrete regions of brain after E2 and MT treatments in male and female catfish, respectively using qPCR which revealed that GnRH1 expression was significantly higher in E2 treated male as compared to the control. On the other hand, GnRH1 expression was lower in MT treated female when compared to the control in the discrete regions of brain. In addition, certain brain and monoaminergic system related genes showed a differential response. Catfish GnRH1 could be localized in preoptic area-hypothalamus (POA-HYP) that correlated with the expression profile in the discrete regions of catfish brain. Serum levels of sex steroids in the treated male fish indicated that the treatment of E2 could maintain and impart feminization effect even in the presence of endogenous androgen during gonadal recrudescence while such an effect was not seen in females with androgen treatment. Measurement of CAs, L-3,4-dihydroxyphenylalanine, dopamine and norepinephrine levels in the male and female brain after the controlled release of E2 and MT, respectively confirmed the modulation of neurotransmitters in the E2treated male than MT treated female fish. These results collectively suggest the severity of estrogenic over androgenic compounds to alter reproductive status even at a minimal dose by targeting CAs and GnRH1 at the level of brain of catfish. This study provides insights into the reproductive toxicity of sex steroid analogues at the level of brain GnRH1 and CA-ergic system in addition to serum T, 11-KT and E2 levels during gonadal recrudescence, which is a crucial period of gametogenesis preceding spawning.