Phytoremediation of anaerobically digested swine wastewater contaminated by oxytetracycline via Lemna aequinoctialis: Nutrient removal, growth characteristics and degradation pathways

• Published in: Bioresource technology 2019-11, Vol.291, p.121853-121853, Article 121853
• Main Authors: Hu, Hao, Zhou, Qi, Li, Xiang, Lou, Wei, Du, Cheng, Teng, Qing, Zhang, Dongmei, Liu, Hongyu, Zhong, Yuanyuan, Yang, Chunping
• Format: Article
• Language: English
• Subjects: anaerobic digestion; Anaerobiosis; Animals; Anti-Bacterial Agents - metabolism; Antibiotic; Araceae - metabolism; Biodegradation, Environmental; Chromatography, Liquid; Duckweed; Lemna aequinoctialis; liquid chromatography; livestock feeds; Nutrients; Oxytetracycline; Oxytetracycline - metabolism; photosynthesis; Phytoremediation; protein content; Swine; Swine wastewater; Tandem Mass Spectrometry; Waste Water - chemistry; wastewater
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.121853

[Display omitted] •Oxytetracycline could significantly inhibit nutrient removal and the duckweed growth.•0.05 mg/L oxytetracycline enhanced photosynthetic pigment and protein synthesis.•The protein content in duckweed gradually decreased with the increased duration.•Twelve intermediates and degradation pathways of oxytetracycline were proposed. The concentration of antibiotics in anaerobically digested swine wastewater (ADSW) usually gradually increases due to the addition of antibiotics in livestock feed. Lemna aequinoctialis was used to treatment synthetic ADSW contaminated by oxytetracycline (OTC) whose concentrations were 0.05, 0.25, 0.50 and 1.00 mg/L, and its influences on NH3-N and TP remove were investigated. The fresh weight, photosynthetic pigment and protein content of duckweed were also investigated. Results have shown that nutrient removal and duckweed growth followed the “dose-response” relationships, and 0.05 mg/L OTC could significantly promote the synthesis of photosynthetic pigments and proteins in duckweed. Meanwhile, the protein content gradually decreased during investigation. More important, the degradation products and possible degradation pathways of OTC were diagrammatized via liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and twelve intermediates were detected in the duckweed systems. This study can offer a novel view for phytoremediation of ADSW containing antibiotics by aquatic plants.