Distribution pattern of small RNA and degradome reads provides information on miRNA gene structure and regulation

Plant microRNAs (miRNAs) have an impact in the regulation of several biological processes such as development, growth and metabolism by negatively controlling gene expression at the post-transcriptional level. However, the role of these small molecules in the symbiotic interaction of plant roots and...

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Bibliographic Details
Published in:Plant signaling & behavior 2011-10, Vol.6 (10), p.1609-1611
Main Authors: Branscheid, Anja, Devers, Emanuel A., May, Patrick, Krajinski, Franziska
Format: Article
Language:English
Subjects:
Addendum
Base Sequence
Binding
Biology
Bioscience
Calcium
Cancer
Cell
Cycle
gene expression
Gene Expression Regulation, Plant
genes
Genetic Loci - genetics
Genome, Plant - genetics
genomics
GRAS substances
high-throughput nucleotide sequencing
Landes
Medicago truncatula
Medicago truncatula - genetics
Medicago truncatula - microbiology
messenger RNA
metabolism
microRNA
MicroRNAs - genetics
Molecular Sequence Data
Mycorrhizae - physiology
mycorrhizal fungi
Organogenesis
Proteins
RNA Stability - genetics
RNA, Plant - genetics
RNA, Plant - metabolism
roots
signal transduction
transcription factors
vesicular arbuscular mycorrhizae
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Summary:Plant microRNAs (miRNAs) have an impact in the regulation of several biological processes such as development, growth and metabolism by negatively controlling gene expression at the post-transcriptional level. However, the role of these small molecules in the symbiotic interaction of plant roots and arbuscular mycorrhizal (AM) fungi remained elusive. To elucidate the role of miRNAs during AM symbiosis we used a deep sequencing approach to analyze the small RNA and degradome sequence tags of Medicago truncatula non-mycorrhizal and mycorrhizal roots. We identified 243 novel Medicago microRNAs and 118 mRNA cleavage targets of miRNA mature and star sequences. Several AM symbiosis-relevant genes were identified as miRNA targets. The transcript of MtNsp2, encoding a GRAS transcription factor involved in the nodule and mycorrhizal signaling pathway, is cleaved by a novel member of the miR171 gene family, namely miR171h. Here, we carried out a detailed analysis of the genomic structure of the MIR171h gene comprising our deep sequencing data. The results suggest a feedback circuit between mature miR171h and its own primary transcript showing the ability of this miRNA regulating itself.
ISSN:1559-2316
1559-2324
1559-2324
DOI:10.4161/psb.6.10.17305