A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo

Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity . Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enz...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2020-06, Vol.11 (23), p.5889-5894
Main Authors: Li, Yuyao, Song, Heng, Xue, Chenghong, Fang, Zhijun, Xiong, Liqin, Xie, Hexin
Format: Article
Language:English
Subjects:
Enzyme activity
Enzymes
Fluorescence
Immobilization
Quinones
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Summary:Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity . Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic system.
ISSN:2041-6520
2041-6539
DOI:10.1039/d0sc01273d