Comparative eye and liver differentially expressed genes reveal monochromatic vision and cancer resistance in the shortfin mako shark (Isurus oxyrinchus)

The shortfin mako, Isurus oxyrinchus is an oceanic pelagic shark found worldwide in tropical and subtropical waters. However, the understanding of its biology at molecular level is still incipient. We sequenced the messenger RNA isolated from eye and liver tissues. De novo transcriptome yielded a to...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2020-11, Vol.112 (6), p.4817-4826
Main Authors: Domingues, Rodrigo R., Mastrochirico-Filho, Vito Antonio, Mendes, Natalia J., Hashimoto, Diogo T., Coelho, Rui, da Cruz, Vanessa Paes, Antunes, Agostinho, Foresti, Fausto, Mendonça, Fernando F.
Format: Article
Language:English
Subjects:
Elasmobranch
Gene ontology
Photoreception
Transcriptomics
Tumor suppression
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The shortfin mako, Isurus oxyrinchus is an oceanic pelagic shark found worldwide in tropical and subtropical waters. However, the understanding of its biology at molecular level is still incipient. We sequenced the messenger RNA isolated from eye and liver tissues. De novo transcriptome yielded a total of 705,940 transcripts. A total of 3774 genes were differentially expressed (DEGs), with 1612 in the eye and 2162 in the liver. Most DEGs in the eye were related to structural and signaling functions, including nonocular and ocular opsin genes, whereas nine out of ten most overexpressed genes in the liver were related to tumor suppression, wound healing, and human diseases. Furthermore, DEGs findings provide insights on the monochromatic shark vision and a repertory of cancer-related genes, which may be insightful to elucidate shark resistance to cancer. Therefore, our results provide valuable sequence resources for future functional and population studies. •De novo transcriptome and differentially expressed genes (DEGs) of eye and liver of the Isurus oxyrinchus was performed in order to obtain a repertory of genes and their functions.•The most common DEGs in the eye were related to structural and signaling functions, including nonocular and ocular opsin genes, revealing a monochromatic vision.•Among the ten most overexpressed genes in the liver, nine were related to tumor suppression, wound healing and human diseases.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2020.08.037