Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts

• Published in: Veterinary parasitology 2020-09, Vol.285, p.109219-109219, Article 109219
• Main Authors: Rodrigues, Caroline Issler, Costa, Diego Mota da, Santos, Acidália Carine Vieira, Batatinha, Maria José Moreira, Souza, Fernanda Vidigal Duarte, Souza, Everton Hilo de, Botura, Mariana Borges, Alves, Clayton Queiroz, Soares, Taliane Leila, Brandão, Hugo Neves
• Format: Article
• Language: English
• Subjects: Ananas - chemistry; Animals; Anthelmintics - chemistry; Anthelmintics - pharmacology; Antioxidant; Bromeliaceae; Gastrointestinal nematodes; Goats; Larva - drug effects; Nematoda - drug effects; Nematode Infections - parasitology; Ovicidal; Ovum - drug effects; p-coumaric acid; Phytochemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Propionates - isolation & purification; Propionates - pharmacology
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2020.109219

•Extracts of BRS Boyrá have in vitro anthelmintic and antioxidant activities;•‘BRS Boyrá’ extracts contains phenolic compounds, including flavonoids;•β-sitosterol, stigmasterol and p-coumaric acid were isolated in BRS Boyrá extracts;•The p-coumaric acid exhibited ovicidal effect against goats nematodes. Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22–14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13–1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16–21.38 mg mL−1 and inhibition of co-oxidation of β-carotene of 36.40–74.86%) and anthelmintic activity (15.69–100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: β-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL−1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive.