Lateral flow assay modified with time-delay wax barriers as a sensitivity and signal enhancement strategy

The ease of use, low cost and quick operation of lateral flow assays (LFA) have made them some of the most common point of care biosensors in a variety of fields. However, their generally low sensitivity has limited their use for more challenging applications, where the detection of low analytic con...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2020-11, Vol.168, p.112559-112559, Article 112559
Main Authors: Sena-Torralba, Amadeo, Ngo, Duy Ba, Parolo, Claudio, Hu, Liming, Álvarez-Diduk, Ruslan, Bergua, José Francisco, Rosati, Giulio, Surareungchai, Werasak, Merkoçi, Arben
Format: Article
Language:English
Subjects:
Biological Assay
Biosensing Techniques
Humans
Immunoglobulin G
Lateral flow
Limit of Detection
Optical biosensor
Point-of-Care
Point-of-Care Systems
Sensitivity enhancement
Wax barrier
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Summary:The ease of use, low cost and quick operation of lateral flow assays (LFA) have made them some of the most common point of care biosensors in a variety of fields. However, their generally low sensitivity has limited their use for more challenging applications, where the detection of low analytic concentrations is required. Here we propose the use of soluble wax barriers to selectively and temporarily accumulate the target and label nanoparticles on top of the test line (TL). This extended internal incubation step promotes the formation of the immune-complex, generating a 51.7-fold sensitivity enhancement, considering the limit of quantification, and up to 96% signal enhancement compared to the conventional LFA for Human IgG (H-IgG) detection. •Easy-to-use and low-cost strategy for sensitivity enhancement in lateral flow assay.•Dissolvable wax barrier printed for the first time after the test line.•51.7-fold sensitivity enhancement for Human IgG detection.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2020.112559