Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus)

The bacteriophage vB_AhM_PVN02 (PVN02), infecting Aeromonas hydrophila , was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively....

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Bibliographic Details
Published in:Archives of virology 2020-12, Vol.165 (12), p.2973-2977
Main Authors: Tu, Vinh Q., Nguyen, Tan-Trung, Tran, Xuan T. T., Millard, Andrew D., Phan, Huyen T., Le, Nga P., Dang, Oanh T. H., Hoang, Hoang A.
Format: Article
Language:English
Subjects:
Aeromonas hydrophila
Annotated Sequence Record
Antibiotic resistance
Biological control
Biomedical and Life Sciences
Biomedicine
Burst size
Genomes
Infectious Diseases
Latent period
Medical Microbiology
Myoviridae
New species
Nucleotide sequence
Phages
Phylogeny
Transmission electron microscopy
Virology
Virulence
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Summary:The bacteriophage vB_AhM_PVN02 (PVN02), infecting Aeromonas hydrophila , was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family Myoviridae . The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family Myoviridae . This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control Aeromonas hydrophila in striped catfish in the Mekong Delta, Vietnam.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-020-04793-2