Toll-like receptors as diagnostic targets in pellucid marginal degeneration

The main purpose of this study is to evaluate the diagnostic role of Toll-like receptors 2 (TLR2) and 4 (TLR4) expression in corneal and conjunctival epithelial cells of eyes with pellucid marginal degeneration (PMD) compared to keratoconus patients (KC) and control subjects. A prospective case-cont...

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Bibliographic Details
Published in:Experimental eye research 2020-11, Vol.200, p.108211-108211, Article 108211
Main Authors: Regueiro, Uxía, Pérez-Mato, María, Hervella, Pablo, Campos, Francisco, Sobrino, Tomás, Lema, Isabel
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Child
Conjunctiva - metabolism
Conjunctiva - physiology
Cornea - metabolism
Cornea - pathology
Corneal ectasia
Corneal Topography
Female
Gene Expression Regulation
Humans
Innate immunity
Keratoconus
Keratoconus - diagnosis
Keratoconus - genetics
Keratoconus - metabolism
Male
Middle Aged
Pellucid marginal degeneration
Prospective Studies
RNA - genetics
ROC Curve
Toll-Like Receptors - biosynthesis
Toll-Like Receptors - genetics
Toll-like receptors 2
Toll-like receptors 4
Young Adult
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Summary:The main purpose of this study is to evaluate the diagnostic role of Toll-like receptors 2 (TLR2) and 4 (TLR4) expression in corneal and conjunctival epithelial cells of eyes with pellucid marginal degeneration (PMD) compared to keratoconus patients (KC) and control subjects. A prospective case-control study in 29 PMD eyes, 109 KC eyes and 72 healthy eyes was done. All participants were subjected to a clinical, topographic, aberrometric and tomographic exam with extraction of corneal and conjunctival epithelial cells through scraping. The TLR2 and TLR4 expression was measured with flow cytometry. Receiver operating characteristic (ROC) curve analysis was used to determine the most appropriate cutoff point for predicting the risk of PMD and KC. Correlations between TLR2/TLR4 expression and the severity of PMD/KC were evaluated. A TLRs follow-up review was made 19 ± 4 months after to the first review. As result, mean expression of TLR2 and TLR4 in both corneal and conjunctival epithelial cells was significantly higher in eyes with corneal ectasia (PMD and KC) than in control eyes (all p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2020.108211