Emergence of multidrug-resistant Shigella species harboring extended-spectrum beta-lactamase genes in pediatric patients with diarrhea from southwest of Iran

• Published in: Molecular biology reports 2020-09, Vol.47 (9), p.7097-7106
• Main Authors: Farajzadeh Sheikh, Ahmad, Moradi Bandbal, Maryam, Saki, Morteza
• Format: Article
• Language: English
• Subjects: Adolescent; Amikacin; Ampicillin; Animal Anatomy; Animal Biochemistry; Antibiotic resistance; Antibiotics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; beta-Lactamases - genetics; beta-Lactamases - metabolism; Biomedical and Life Sciences; Cefepime; Cefotaxime; Ceftazidime; Ceftriaxone; Child; Child, Preschool; Cotrimoxazole; Cross-Sectional Studies; Diarrhea; Diarrhea - enzymology; Diarrhea - genetics; Diarrhea - microbiology; Erythromycin; Female; Histology; Humans; Imipenem; Iran; Life Sciences; Male; Morphology; Multidrug resistance; Multidrug resistant organisms; Original Article; Patients; Pediatrics; Polymerase chain reaction; Serotyping; Shigella; Shigella - enzymology; Shigella - genetics; Species; β Lactamase
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-05776-x

Owing to the scarce evidence about the multidrug-resistant (MDR) beta-lactamase-producing Shigella isolates in Iran, this study aimed to evaluate the occurrence of extended-spectrum beta-lactamases (ESBL) and AmpC β-lactamases in Shigella species collected in the southwest of Iran. This study was conducted on Shigella species isolated from stool samples of pediatric patients aged less than 15 years suffering from diarrhea. These isolates were identified by bacteriology tests, serotyping, and polymerase chain reaction (PCR). The antibiotic resistance was determined by disc diffusion. The production of ESBLs and AmpC was investigated by phenotypic confirmatory tests and PCR. In total, 79 Shigella isolates, including 46.8% (n = 37) of S. flexneri and 53.2% (n = 42) of S. sonnei , were isolated, respectively. The most effective antibiotic was imipenem with 93.7% of susceptibility followed by ampicillin (29.1%), and cotrimoxazole (30.4%).The resistance rates of ceftriaxone, ceftazidime, and cefotaxime were 41.8%, 34.2%, and 41.8%, respectively. Also, a total of 57 (72.2%) isolates showed MDR profiles. The phenotypic tests showed that 43.0% (34/79) of isolates can produce ESBLs, and no one was positive for ApmC. The frequency of bla TEM and bla CTX-M were 30.4% and 32.9%, respectively, while the bla PER , bla SHV, and AmpC genes were not detected. The ESBL-producing isolates had a significant ( p -value ˂ 0.05) resistance rate against ceftriaxone, ceftazidime, cefotaxime, cefepime, erythromycin, and amikacin. The significant prevalence of MDR Shigella isolates harboring ESBL genes highlights the need for effective surveillance measures to prevent the more spread of drug resistance among species.