Molecular epidemiology and antifungal susceptibilities of Cryptococcus species isolates from HIV and non-HIV patients in Southwest China

To investigated the molecular epidemiology and in vitro antifungal susceptibility of Cryptococcus isolates from West China Hospital from HIV and non-HIV patients between 2009 and 2015. A total of 132 C. neoformans and C. gattii were subjected to antifungal susceptibility testing by E -test method. A...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2021-02, Vol.40 (2), p.287-295
Main Authors: Wu, Si-Ying, Kang, Mei, Liu, Ya, Chen, Zhi-Xing, Xiao, Yu-Ling, He, Chao, Ma, Ying
Format: Article
Language:English
Subjects:
Amphotericin B
Antifungal agents
Biomedical and Life Sciences
Biomedicine
Cryptococcus
Cryptococcus neoformans
Epidemiology
Fluconazole
Flucytosine
Fungal infections
Fungi
Fungicides
Genetic diversity
HIV
Human immunodeficiency virus
Internal Medicine
Itraconazole
Medical Microbiology
Minimum inhibitory concentration
Multilocus sequence typing
Original Article
Phenotypes
Susceptibility
Test methods
Voriconazole
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Summary:To investigated the molecular epidemiology and in vitro antifungal susceptibility of Cryptococcus isolates from West China Hospital from HIV and non-HIV patients between 2009 and 2015. A total of 132 C. neoformans and C. gattii were subjected to antifungal susceptibility testing by E -test method. Among the 132 isolates, 42 C. neoformans and C. gattii were analyzed by mating type and URA5 -RFLP. A total of 113 C. neoformans and C. gattii were subjected to multi-locus sequence typing (MLST). MLST results revealed that ST5 was the major molecular type. The wild-type (WT) phenotype was seen in 91.5–100% of C. neoformans isolates for amphotericin B, 5-flucytosine, fluconazole, and voriconazole. However, 72.3% (94/130) of C. neoformans isolates were non-wild-type (non-WT) to itraconazole by E -test method. In the sixth study year, the geometric mean, MIC 50 and MIC 90 of fluconazole were the highest ( P  
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-020-04013-4