Peculiarities of Cell Seeding on Electroformed Polycaprolactone Scaffolds Modified with Surface-Active Agents Triton X-100 and Polyvinylpyrrolidone

• Published in: Bulletin of experimental biology and medicine 2020-08, Vol.169 (4), p.600-604
• Main Authors: Afanasiev, S. A., Muslimova, E. F., Nashchekina, Yu. A., Nikonov, P. O., Rogovskaya, Yu. V., Tenchurin, T. Kh, Nesterenko, E. V., Grakova, E. V., Kopeva, K. V., Akhmedov, S. D.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell Biology; Internal Medicine; Laboratory Medicine; Pathology; Povidone; Surface active agents; Tissue engineering; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-020-04936-0

We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Х-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2.19 and 2.46±2.15 μ, respectively. Type 1 scaffold had higher surface density and hydrophilicity, when type 2 scaffold was 1.6 times thicker. The cells were seeded on the scaffolds by the dynamic seeding technique and then cultured in Petri dishes with nutrient medium in a humid atmosphere. During 3-day culturing, no cell release from the matrix was noted. DAPI staining proved the presence of cells in both scaffolds. However, in type 1 scaffold the cells populated the whole thickness, while in type 2 scaffold, the cells were present only in the superficial layer. These findings suggest that PCL scaffolds modified with Triton Х-100 or polyvinylpyrrolidone are not cytotoxic, but the structure of the scaffold treated with Triton Х-100 is more favorable for population with cells.