Super-resolved edge detection in optical microscopy images by superposition of virtual point sources

A new approach to the edge detection problem is presented which is specially designed to achieve high accuracy detection, below instrumental resolution (super resolution) in microscopy images. The method is based in a modified version of a recently published algorithm known as SUPPOSe, which perform...

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Bibliographic Details
Published in:Optics express 2020-08, Vol.28 (17), p.25319-25334
Main Authors: Brinatti Vazquez, Guillermo D., Martínez, Sandra, Martínez, Oscar E.
Format: Article
Language:English
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Summary:A new approach to the edge detection problem is presented which is specially designed to achieve high accuracy detection, below instrumental resolution (super resolution) in microscopy images. The method is based in a modified version of a recently published algorithm known as SUPPOSe, which performs a numerical reconstruction of an image as a superposition of virtual point sources. The method was tested in simulated and experimental optical microscopy images and compared to the standard Laplacian of Gaussian algorithm, showing huge differences when the size of the object is smaller than the lateral resolution provided by the instrument.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.397125