A Fluorescence Resonance Energy Transfer Biosensor Based on Graphene Quantum Dots and Protoporphyrin IX for the Detection of Melamine

Graphene quantum dots (GQDs), which have high photostability, anti-photobleaching and scintillation, good biocompatibility and low toxicity, are important member of the fluorescent material family, and have attracted extensive research interest. In this paper, a fluorescence resonance energy transfe...

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Bibliographic Details
Published in:Journal of fluorescence 2020-12, Vol.30 (6), p.1463-1468
Main Authors: Xue, Gao, Zhiying, Ma, Xiuying, Liu, Lijun, Tang, Jianrong, Li
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biocompatibility
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biosensors
Biotechnology
Energy transfer
Fluorescence
Graphene
Melamine
Milk
Original Article
Quantum dots
Resonance
Self-assembly
Shutdowns
Toxicity
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Summary:Graphene quantum dots (GQDs), which have high photostability, anti-photobleaching and scintillation, good biocompatibility and low toxicity, are important member of the fluorescent material family, and have attracted extensive research interest. In this paper, a fluorescence resonance energy transfer (FRET) biosensor based on protoporphyrin IX (PpIX) and GQDs was developed for melamine detection. PpIX was bound to the surface of GQDs to produce self-assembled nanosensors, and a FRET process occurred between GQDs and PpIX. However, due to the combination of melamine and PpIX, the FRET process was shut down in the presence of melamine. The FRET system could quickly and accurately detect melamine with a detection range of 1.0 × 10 −8 to 2.0 × 10 −6  mol/L based on the fluorescence intensity ratio of PpIX and GQDs, and the detection limit was 3.6 × 10 −9  mol/L. This method obtained satisfactory results when it was employed to the determination of melamine in milk samples.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-020-02524-z