Purification of the phytase enzyme from Lactobacillus plantarum: The effect on pansy growth and macro–micro element content

In the present study, the phytase enzyme was purified from Lactobacillus plantarum with a 3.08% recovery, 9.57‐purification fold, and with a specific activity of 278.82 EU/mg protein. Then, the effects of the 5 EU and 10 EU purified phytase was determined on the plant growth, quality, the macro–micr...

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Published in:Biotechnology and applied biochemistry 2021-10, Vol.68 (5), p.1067-1075
Main Authors: Karagöz, Fazilet Parlakova, Demir, Yeliz, Kotan, Merve Şenol, Dursun, Atilla, Beydemir, Şükrü, Dikbaş, Neslihan
Format: Article
Language:English
Subjects:
6-Phytase - isolation & purification
6-Phytase - metabolism
enzyme purification
Enzymes
Lactobacilli
Lactobacillus plantarum
Lactobacillus plantarum - enzymology
Natural lighting
Nutrient content
Nutrients - analysis
Ornamental plants
Peat
Phytase
Plant growth
plant growth, Viola × wittrockiana
Protein folding
Purification
Seedlings
Seeds
Seeds - chemistry
Seeds - metabolism
Viola - chemistry
Viola - growth & development
Viola - metabolism
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Summary:In the present study, the phytase enzyme was purified from Lactobacillus plantarum with a 3.08% recovery, 9.57‐purification fold, and with a specific activity of 278.82 EU/mg protein. Then, the effects of the 5 EU and 10 EU purified phytase was determined on the plant growth, quality, the macro–micro nutrient content of pansy (Viola × wittrockiana), which is of great importance in ornamental plants industry. The research was established under greenhouse conditions with natural light in 2017. The pansy seeds were coated with phytase enzyme solution, sown in a peat environment, and transferred to pots at the seedling period. In general, the 5 EU and 10 EU applications increase plant height, the number of leaves per plant, the number of side branches per plant, and flower height parameters compared to control. Also, micro‐ and macronutrient values in soil and plant samples were examined. According to the results, the phytase application on pansy cultivation positively affected the properties and yielded high quality of plants. The effect of phytase applications on potted ornamental plant quality: (A) control, (B) phytase (5 EU), and (C) phytase (10 EU).
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2026