PTPN13 acts as a tumor suppressor in clear cell renal cell carcinoma by inactivating Akt signaling

• Published in: Experimental cell research 2020-11, Vol.396 (1), p.112286-112286, Article 112286
• Main Authors: Long, Qingzhi, Sun, Jiping, Lv, Jia, Liang, Yu, Li, Huixian, Li, Xudong
• Format: Article
• Language: English
• Subjects: Akt; Animals; Apoptosis - genetics; Carcinoma, Renal Cell - genetics; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - mortality; Carcinoma, Renal Cell - pathology; ccRCC; Cell Line; Cell Line, Tumor; Cell Proliferation; Epithelial Cells - metabolism; Epithelial Cells - pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Kidney Neoplasms - genetics; Kidney Neoplasms - metabolism; Kidney Neoplasms - mortality; Kidney Neoplasms - pathology; Mice; Mice, Inbred BALB C; Mice, Nude; Protein Tyrosine Phosphatase, Non-Receptor Type 13 - antagonists & inhibitors; Protein Tyrosine Phosphatase, Non-Receptor Type 13 - genetics; Protein Tyrosine Phosphatase, Non-Receptor Type 13 - metabolism; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; PTPN13; Renal cell carcinoma; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Signal Transduction - genetics; Survival Analysis; Tumor Burden; Xenograft Model Antitumor Assays
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2020.112286

Protein tyrosine phosphatase, nonreceptor type 13 (PTPN13), has emerged as a critical cancer-related gene that is implicated in a wide range of cancer types. However, the role of PTPN13 in clear cell renal cell carcinoma (ccRCC) is poorly understood. In the present study, we aimed to evaluate whether PTPN13 participates in the progression of ccRCC. Decreased expression of PTPN13 was found in ccRCC tissues, which predicted a shorter survival rate in ccRCC patients. PTPN13 expression was also lower in ccRCC cell lines, and the upregulation of PTPN13 repressed the proliferation, colony formation and invasion, but enhanced the apoptosis of ccRCC cells. In contrast, the silencing of PTPN13 produced the opposite effects. Further data showed that PTPN13 overexpression decreased the phosphorylation of Akt, while PTPN13 silencing increased the phosphorylation of Akt. Treatment with Akt inhibitor markedly abrogated the PTPN13 silencing-evoked oncogenic effect in ccRCC cells. Xenograft tumor experiments revealed that overexpression of PTPN13 remarkably restricted the tumor formation and growth of ccRCC cells in vivo associated with inactivation of Akt. In conclusion, our data demonstrated that overexpression of PTPN13 restricts the proliferation and invasion of ccRCC cells through inactivation of Akt. Our study suggests a tumor suppressive function of PTPN13 in ccRCC and highlights the potential role of PTPN13 in the progression of ccRCC. [Display omitted] •PTPN13 is decreased in ccRCC.•PTPN13 exerts a tumor suppressive role in ccRCC.•PTPN13 inhibits Akt activation.•PTPN13 inhibits the progression of ccRCC by inhibiting Akt.