A set of rhamnosylation-specific antibodies enables detection of novel protein glycosylations in bacteria

Despite its potential importance for bacterial virulence, protein rhamnosylation has not yet been sufficiently studied. Specific anti -Ser Rha , anti -Thr Rha and anti -Asn Rha antibodies allowed the identification of previously unknown monorhamnosylated proteins in cytosol and membrane fractions of...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2020-09, Vol.18 (35), p.6823-6828
Main Authors: Gast, Daniel, Koller, Franziska, Krafczyk, Ralph, Bauer, Lukas, Wunder, Swetlana, Lassak, Jürgen, Hoffmann-Röder, Anja
Format: Article
Language:English
Subjects:
Antibodies
Bacteria
Bacterial diseases
Bacterial Proteins - chemistry
Bacterial Proteins - immunology
Bacterial Proteins - metabolism
Cytosol
Glycosylation
Lysates
Peptide mapping
Proteins
Rhamnose - chemistry
Virulence
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Summary:Despite its potential importance for bacterial virulence, protein rhamnosylation has not yet been sufficiently studied. Specific anti -Ser Rha , anti -Thr Rha and anti -Asn Rha antibodies allowed the identification of previously unknown monorhamnosylated proteins in cytosol and membrane fractions of bacterial cell lysates. Mapping of the complete rhamnoproteome in pathogens should facilitate development of targeted therapies against bacterial infections. Chemical curiosity or not? Specific antibodies to study the rhamnoproteome in bacteria.
ISSN:1477-0520
1477-0539
1477-0539
DOI:10.1039/d0ob01289k