Comparative analysis of the pulmonary microbiome in healthy and diseased pigs

• Published in: Molecular genetics and genomics : MGG 2021, Vol.296 (1), p.21-31
• Main Authors: Li, Zongjie, Wang, Xin, Di, Di, Pan, Ruyi, Gao, Yun, Xiao, Changguang, Li, Beibei, Wei, Jianchao, Liu, Ke, Qiu, Yafeng, Ma, Zhiyong
• Format: Article
• Language: English
• Subjects: Alveoli; Animal Genetics and Genomics; Animals; Biochemistry; Biomedical and Life Sciences; Bordetella - classification; Bordetella - genetics; Bordetella - isolation & purification; Bordetella - pathogenicity; Bronchoalveolar Lavage Fluid - microbiology; Bronchus; Comparative analysis; DNA, Bacterial - genetics; Enterococcus - classification; Enterococcus - genetics; Enterococcus - isolation & purification; Genera; Haemophilus - classification; Haemophilus - genetics; Haemophilus - isolation & purification; Haemophilus - pathogenicity; High-Throughput Nucleotide Sequencing; Human Genetics; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactococcus - classification; Lactococcus - genetics; Lactococcus - isolation & purification; Life Sciences; Lung - microbiology; Lung diseases; Microbial Genetics and Genomics; Microbiomes; Microbiota; Microbiota - genetics; Original Article; Pasteurella - classification; Pasteurella - genetics; Pasteurella - isolation & purification; Pasteurella - pathogenicity; Phylogeny; Plant Genetics and Genomics; Respiration; Respiratory diseases; Respiratory tract diseases; Respiratory Tract Infections - microbiology; RNA, Ribosomal, 16S - genetics; Staphylococcus - classification; Staphylococcus - genetics; Staphylococcus - isolation & purification; Streptococcus - classification; Streptococcus - genetics; Streptococcus - isolation & purification; Streptococcus - pathogenicity; Streptococcus infections; Swine - microbiology
• ISSN: 1617-4615; 1617-4623
• DOI: 10.1007/s00438-020-01722-5

The lungs possess an effective antimicrobial system and a strong ability to eliminate microorganisms in healthy organisms, and were once considered sterile. With the development of culture-independent sequencing technology, the richness and diversity of porcine lung microbiota have been gaining attention. In order to study the relationship between lung microbiota and porcine respiratory disease complex (PRDC), the lung microbiota in healthy and diseased swine bronchoalveolar lavage fluids were analyzed and compared using the Illumina MiSeq sequencing platform. The predominant microbial communities of healthy and diseased swine were similar at the phylum level, mainly composed of Proteobacteria, Firmicutes, Tenericutes, and Bacteroidetes. However, the bacterial taxonomic communities of healthy and diseased swine differed at the genus level. The higher relative abundances of Lactococcus , Enterococcus , Staphylococcus, and Lactobacillus genera in healthy swine might provide more benefits for lung health, while the enhanced richness of Streptococcus , Haemophilus , Pasteurella, and Bordetella genera in diseased swine might be closely related to pathogen invasion and the occurrence of respiratory disease. In conclusion, the observed differences in the richness and diversity of lung microbiota can provide novel insights into their relationship with PRDC. Analyses of swine lung microbiota communities might produce an effective strategy for the control and prevention of respiratory tract infections.