Essential role of Cav3.2 T-type calcium channels in butyrate-induced colonic pain and nociceptor hypersensitivity in mice

• Published in: European journal of pharmacology 2020-11, Vol.887, p.173576-173576, Article 173576
• Main Authors: Tsubota, Maho, Matsui, Kazuki, Nakano, Maki, Kajitani, Rie, Ishii, Yuko, Tomochika, Ken, Nishikawa, Yuta, Fukushi, Saaya, Yamagata, Ayumu, Sekiguchi, Fumiko, Okada, Takuya, Toyooka, Naoki, Kawabata, Atsufumi
• Format: Article
• Language: English
• Subjects: Butyrate; Cav3.2 T-type calcium channel; Colonic pain; Irritable bowel syndrome; Nociceptor hypersensitivity
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2020.173576

Given the role of Cav3.2 isoform among T-type Ca2+ channels (T-channels) in somatic and visceral nociceptive processing, we analyzed the contribution of Cav3.2 to butyrate-induced colonic pain and nociceptor hypersensitivity in mice, to evaluate whether Cav3.2 could serve as a target for treatment of visceral pain in irritable bowel syndrome (IBS) patients. Mice of ddY strain, and wild-type and Cav3.2-knockout mice of a C57BL/6J background received intracolonic administration of butyrate twice a day for 3 days. Referred hyperalgesia in the lower abdomen was assessed by von Frey test, and colonic hypersensitivity to distension by a volume load or chemicals was evaluated by counting nociceptive behaviors. Spinal phosphorylated ERK was detected by immunohistochemistry. Cav3.2 knockdown was accomplished by intrathecal injection of antisense oligodeoxynucleotides. Butyrate treatment caused referred hyperalgesia and colonic hypersensitivity to distension in ddY mice, which was abolished by T-channel blockers and/or Cav3.2 knockdown. Butyrate also increased the number of spinal phosphorylated ERK-positive neurons following colonic distension in the anesthetized ddY mice. The butyrate-treated ddY mice also exhibited T-channel-dependent colonic hypersensitivity to intracolonic Na2S, known to enhance Cav3.2 activity, and TRPV1, TRPA1 or proteinase-activated receptor 2 (PAR2) agonists. Wild-type, but not Cav3.2-knockout, mice of a C57BL/6J background, after treated with butyrate, mimicked the T-channel-dependent referred hyperalgesia and colonic hypersensitivity in butyrate-treated ddY mice. Our study provides definitive evidence for an essential role of Cav3.2 in the butyrate-induced colonic pain and nociceptor hypersensitivity, which might serve as a target for treatment of visceral pain in IBS patients. •Intracolonic butyrate caused colonic pain and hypersensitivity to distention in mice.•Butyrate-treated mice exhibited colonic hypersensitivity to nociceptor stimulation.•Butyrate-induced colonic pain and hypersensitivity require Cav3.2 T-channel activity.•Cav3.2 is considered a promising target for treatment of colonic pain symptoms.