Cryptosporidium parvum - zoonotic subtype IIdA15G1 in a Slovakian patient

INTRODUCTION AND OBJECTIVESThe parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptospor...

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Published in:Annals of agricultural and environmental medicine : AAEM 2020, Vol.27 (3), p.485-488
Main Authors: Mravcová, Kristína, Štrkolcová, Gabriela, Mucha, Rastislav, Barbušinová, Eva, Goldová, Mária, Kačírová, Jana, Maďar, Marián
Format: Report
Language:English
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Summary:INTRODUCTION AND OBJECTIVESThe parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. MATERIAL AND METHODSThe Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. RESULTSThe nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. CONCLUSIONSThis is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.
ISSN:1898-2263
DOI:10.26444/aaem/126619