Luteolibacter luteus sp. nov., isolated from stream bank soil

• Published in: Archives of microbiology 2021, Vol.203 (1), p.377-382
• Main Authors: Dahal, Ram Hari, Chaudhary, Dhiraj Kumar, Kim, Dong-Uk, Kim, Jaisoo
• Format: Article
• Language: English
• Subjects: Amino acids; Base Composition; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Casein; Catalase; Cell Biology; Deoxyribonucleic acid; Diagnostic systems; Diaminopimelic Acid - chemistry; DNA; DNA, Bacterial - genetics; Ecology; Esculin; Fatty acids; Fatty Acids - chemistry; Gene clusters; Genomes; Gram stain; Life Sciences; Lipids; Menaquinones; Metabolites; Microbial Ecology; Microbiology; New species; Original Paper; Peptidoglycans; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phospholipids - chemistry; Phylogenetics; Phylogeny; Quinones; Republic of Korea; Rivers; RNA, Ribosomal, 16S - genetics; rRNA 16S; Secondary metabolites; Soil Microbiology; Soils; Species Specificity; Stream banks; Verrucomicrobia - classification; Verrucomicrobia - genetics
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-020-02048-x

A non-motile, Gram-stain-negative, rod-shaped and yellow-colored bacterium, designated G-1-1-1 T was obtained from soil sampled at Gwanggyo stream bank, Gyeonggi-do, Republic of Korea. Cells were aerobic, catalase positive, grew optimally at 25–30 °C and hydrolysed aesculin and casein. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-1-1-1 T formed a lineage within the genus Luteolibacter . The closest members were Luteolibacter flavescens GKX T (97.7% sequence similarity) and Luteolibacter arcticus MC 3726 T (97.3%). The sequence similarities with other members of the genus Luteolibacter were ≤ 93.9%. The genome of strain G-1-1-1 T was 6,412,079 bp long with 5176 protein-coding genes. The diagnostic amino acid of cell-wall peptidoglycan of strain G-1-1-1 T was meso -diaminopimelic acid. The only respiratory quinone was menaquinone-9 and the principal polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. The predominant cellular fatty acids were iso-C 14:0 , C 16:1 ω 9 c , C 16:0 , C 14:0 and anteiso-C 15:0 . The DNA G + C content was 61.0 mol%. The anti-SMASH analysis of whole genome showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain G-1-1-1 T represents a novel species in the genus Luteobacter , for which the name Luteolibacter luteus sp. nov. is proposed. The type strain is G-1-1-1 T (= KACC 21614 T  = NBRC 114341 T ).