Sensitive determination of NT‐proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor

Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health‐related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its conditio...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2021-08, Vol.68 (4), p.865-870
Main Authors: Qiu, Changcheng, Wang, Xiao, Zhang, Xia, Li, Zhenhuan, Zhou, Yiming, Kang, Jie
Format: Article
Language:English
Subjects:
abdominal aortic aneurysm
Aneurysms
Aorta
Aortic aneurysms
Aptamers
Biomarkers
biosensor
Cardiovascular diseases
Cardiovascular system
Coronary vessels
dielectrode
Electrodes
Health risks
Heart
Risk analysis
Risk factors
Surgery
vascular disease
Vascular diseases
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Summary:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health‐related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immediate treatment. Blood‐based biomarker helps to diagnose AAA and to monitor the condition after AAA surgery. N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) is a hormone produced in the heart in small quantities and increased when the heart needs to work harder. NT‐proBNP was proved to be strongly linked with AAA incidence. Moreover, quantifying the level of NT‐proBNP helps to determine the risk factors on cardiovascular system after the surgery. This work is quantifying the NT‐proBNP on interdigitated electrode sensor by using NT‐proBNP binding aptamer. The detection limit of NT‐proBNP was calculated as 1 pg/mL on a linear regression curve [y = 0.2148x + 0.8849; R² = 0.9049]. The linear range with dose‐dependent analysis was from 0.01 until 100 ng/mL. Moreover, the control experiment with complementary aptamer sequence did not show the current signal, specifying the detection of NT‐proBNP. This research benefits to identify the heart condition of patient after the removal of AAA. Diagnosing abdominal aortic aneurysms incidence on inderdigitated electrode sensor.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2006