Effect of chlorogenic acid on alleviating inflammation and apoptosis of IPEC-J2 cells induced by deoxyniyalenol

• Published in: Ecotoxicology and environmental safety 2020-12, Vol.205, p.111376-111376, Article 111376
• Main Authors: Xu, Xiaoxiang, Chang, Juan, Wang, Ping, Yin, Qingqiang, Liu, Chaoqi, Li, Maolong, Song, Andong, Zhu, Qun, Lu, Fushan
• Format: Article
• Language: English
• Subjects: Apoptosis; Chlorogenic acid; Deoxynivalenol; Inflammation; IPEC-J2 cells
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.111376

Deoxynivalenol (DON) is extensively detected in many kinds of foods and feeds to harm human and animal health. This research aims to investigate the effect of chlorogenic acid (CGA) on alleviating inflammation and apoptosis of swine jejunal epithelial cells (IPEC-J2) triggered by DON. The results demonstrated that cell viability was decreased when DON concentrations increased or incubation time expanded. The pretreatment with CGA (40 μg/mL) for 1 h increased cell viability, decreased lactate dehydrogenase (LDH) release and apoptosis in cells triggered by DON at 0.5 μg/mL for 6 h, compared with the DON alone-treated cells. Moreover, the mRNA abundances of IL-8, IL-6, TNF-α, COX-2, caspase-3, Bax and ASCT2 genes, and protein expressions of COX-2, Bax and ASCT2 were significantly down-regulated; while the mRNA abundances of ZO-1, claudin-1, occludin, PePT1 and GLUT2 genes, and protein expressions of ZO-1, claudin-1 and PePT1 were significantly up-regulated in the CGA + DON group, compared with the DON alone group. This study indicated that CGA pretreatment alleviated cytotoxicity, inflammation and apoptosis in DON-triggered IPEC-J2 cells, and protected intestinal cell integrity from DON damages. •Chlorogenic acid (CGA) alleviated deoxynivalenol (DON)-induced IPEC-J2 cytotoxicity.•CGA down-regulated mRNA abundances of cell inflammation cytokines induced by DON.•CGA up-regulated mRNA abundances of cell tight junction protein induced by DON.•CGA altered nutrient-transporter gene mRNA abundances in DON-induced IPEC-J2 cells.•mRNA abundances of relative genes corresponded with their protein expressions.