Effects of a surface prereacted glass–ionomer filler coating material on biofilm formation and inhibition of dentin demineralization

• Published in: Clinical oral investigations 2021-02, Vol.25 (2), p.683-690
• Main Authors: Yamamoto, Shiori, Sayed, Mahmoud, Takahashi, Motoi, Matin, Khairul, Hiraishi, Noriko, Nikaido, Toru, Burrow, Michael F., Tagami, Junji
• Format: Article
• Language: English
• Subjects: Bacteria; Biofilms; Coatings; Demineralization; Dental Caries; Dentin; Dentistry; Glass Ionomer Cements - pharmacology; Humans; Medicine; Mineralization; Original Article; Protective coatings; Statistical analysis; Streptococcus infections; Streptococcus mutans; Tooth Demineralization - prevention & control
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-020-03577-x

Objectives This study investigated the ability of a surface prereacted glass–ionomer (S-PRG) coating material to inhibit the biofilm formation and demineralization of dentin. Methods and materials Dentin specimens were randomly divided into three groups: (1) no coating (control), (2) S-PRG filler-containing coat, and (3) a nonS-PRG filler–containing coat. Streptococcus mutans biofilms were grown on the dentin surfaces in a microcosm for 20 h. Then, the quantity of bacteria and water-insoluble glucan in the retained biofilm on the dentin surface were measured. Regarding demineralization inhibition test, specimens were demineralized for 5 days then sectioned into halves and observed under confocal laser scanning microscope (CLSM). One-way ANOVA and Tukey’s HSD were used for statistical analysis. Results The estimated mean surface roughness for specimens in the S-PRG group was statistically significantly higher than the estimates for both the nonS-PRG and the control group specimens. The quantity of bacteria and water-insoluble glucan/mm 2 revealed that the S-PRG group prevented biofilm formation and bacterial adhesion to the dentin surface compared with the control and nonS-PRG groups. The S-PRG group recorded the highest acid-resistance ability with no surface loss. Conclusion Application of S-PRG barrier coat on dentin surfaces can inhibit biofilm formation as well as protecting the dentin surface against demineralization. Clinical significance Coating material containing S-PRG fillers might be used for caries prevention, through inhibiting biofilm formation, enhancing mineralization, and reducing acidic attack by cariogenic bacteria.