Endothelial Colony-Forming Cells from Idiopathic Pulmonary Fibrosis Patients Have a High Procoagulant Potential

Idiopathic pulmonary fibrosis (IPF) is a severe, progressive and irreversible lung disease constantly associated with a major vascular remodeling process. Endothelial colony-forming cells (ECFCs) are human vasculogenic cells proposed as a cell therapy product or liquid biopsy in vascular disorders....

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Bibliographic Details
Published in:Stem cell reviews and reports 2021-04, Vol.17 (2), p.694-699
Main Authors: Billoir, Paul, Blandinières, Adeline, Gendron, Nicolas, Chocron, Richard, Gunther, Sven, Philippe, Aurélien, Guerin, Coralie L., Israël-Biet, Dominique, Smadja, David M.
Format: Article
Language:English
Subjects:
Anticoagulants
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biopsy
Cell Biology
Cell therapy
Colonies
Colony-forming cells
Cord blood
Endothelial Cells - cytology
Fibrosis
Flow cytometry
Humans
Idiopathic Pulmonary Fibrosis
Life Sciences
Lung diseases
Peripheral blood
Pulmonary fibrosis
Regenerative Medicine/Tissue Engineering
Stem Cells
Stem Cells - cytology
Thrombin
Thrombomodulin
Thrombosis
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Summary:Idiopathic pulmonary fibrosis (IPF) is a severe, progressive and irreversible lung disease constantly associated with a major vascular remodeling process. Endothelial colony-forming cells (ECFCs) are human vasculogenic cells proposed as a cell therapy product or liquid biopsy in vascular disorders. Since the link between IPF and thrombosis has been largely proposed, the aim of our study was to explore hypercoagulability states in ECFCs from patients with IPF. We performed Thrombin generation assay (TGA) in cord blood (CB)-ECFCs, peripheral blood (PB)-ECFCs and IPF-ECFCs. Endogenous thrombin potential and peak were higher in IPF-ECFCs compared to CB-ECFCs and PB-ECFCs. As thrombin generation in ECFCs was increased, we evaluated anticoagulant proteins expressed on ECFCs membrane and identified thrombomodulin and EPCR. We found a significant decrease of both anticoagulant proteins at membrane using flow cytometry. This study is the first to examine ECFC thrombin generation in IPF. This new finding strongly argues for a role of ECFC in IPF pathophysiology and thrombotic related disorders in IPF. Graphical Abstract
ISSN:2629-3269
2629-3277
DOI:10.1007/s12015-020-10043-4