Effect of mechanical injury and IL-1β on the expression of LOXs and MMP-1, 2, 3 in PCL fibroblasts after co-culture with synoviocytes

• Published in: Gene 2021-01, Vol.766, p.145149-145149, Article 145149
• Main Authors: Xu, Chunming, Chi, Qingjia, Yang, Li, Paul Sung, K.L., Wang, Chunli
• Format: Article
• Language: English
• Subjects: Co-culture; LOXs; MMPs; PCL; Synovial cell
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2020.145149

•Injury alone elevated the expression of LOXs and MMP-1, 2 and 3.•IL-1β decreased the LOX expression, and increased MMP expression in injured PCLfs.•Co-culture further suppressed LOXs, but stimulated MMP expressions.•SCs could affect the IL-1β-induction of LOXs inhibition and MMPs accumulation. Crosstalk between posterior cruciate ligament fibroblasts (PCLfs) and synoviocytes (SCs) significantly modifies the homeostatic balance of the extracellular matrix (ECM) and appears to post a prominent affection for wound healing of PCL. Interleukin-1β (IL-1β) is regarded as a critical factor in acute inflammatory events during ligament injury. In order to confirm the capability of SCs the response of lysyl oxidases (LOXs) and matrix metalloproteinases (MMPs) to IL-1β, the complex cues of the joint cavity following PCL injury were simulated and the effect of IL-1β on the expression of LOXs and MMPs in PCLfs were investigated. PCLfs in both the mono- and co-culture conditions were treated with IL-1β. Cell lysates were collected from the PCLfs and LOXs and MMP-1, 2, 3 expression quantified using quantitative real-time PCR and western bolting. The results indicated that injury alone elevated the expression of LOXs and MMP-1, 2 and 3. But IL-1β significantly decreased the LOX, LOXL1, and LOXL3 expression, and simultaneously increased MMP-1, 2 and 3 expressions in injured PCLfs. Furthermore, co-culture further suppressed LOXs, but stimulated MMP-1, 2 and 3 expressions when subjected to both mechanical injury and IL-1β treatment. This possibly suggests that a number of soluble factors are secreted that act as mediators that amplify the response of SCs. The results indicated that the SCs could affect the IL-1β-induction of LOXs inhibition and MMPs accumulation, which may be the underlying mechanism of the the poor healing response following PCL injury.