Development of a Recombinant Monospecific Anti-PLGF Bivalent Nanobody and Evaluation of it in Angiogenesis Modulation

During the past two decades, tumor therapy based on monoclonal antibody has been found as a confident therapeutic approach in solid tumors and hematologic malignancies. Nanobodies are the smallest fragment of an antigen-binding domain in heavy chain-only antibody originated from the Camelidae family...

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Bibliographic Details
Published in:Molecular biotechnology 2020-12, Vol.62 (11-12), p.580-588
Main Authors: Nikooharf, Abolfazl, Arezumand, Roghaye, Mansouri, Kamran, Khoshi, Amir Hossein, Namdar Ahmadabad, Hassan
Format: Article
Language:English
Subjects:
Biochemistry
Biological Techniques
Biotechnology
Cell Biology
Chemistry
Chemistry and Materials Science
Human Genetics
Original Paper
Protein Science
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Summary:During the past two decades, tumor therapy based on monoclonal antibody has been found as a confident therapeutic approach in solid tumors and hematologic malignancies. Nanobodies are the smallest fragment of an antigen-binding domain in heavy chain-only antibody originated from the Camelidae family. Accordingly, they are being recently developed rapidly as diagnostic and therapeutic agents. In this regard, targeting of angiogenic factors like Placenta growth factor (PLGF) via nanobodies show a high effectiveness. In the current study, we developed a recombinant anti-PLGF bivalent nanobody based on the affinity enhancement mutant form of anti-PLGF nanobody to suppress the angiogenesis progression. Thereafter, the bivalent nanobody (bi-Nb) was cloned and then expressed into a bacterial system. Afterward, the purity was authorized using western blot assay and the affinity was assessed using ELISA. In this regard, proliferation, 3D capillary tube formation, and migration assays were employed as functional assays. The obtained data were analyzed using t -test and P  
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-020-00275-7